FastQCFastQC Report
Thu 26 May 2016
ERR580038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5745402
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61910.10775573232299498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75800.042.9937671
GTATCAA126650.038.467681
CAACGCA141050.032.932385
ATCAACG146200.031.9067863
TATCAAC157950.030.5172732
AACGCAG161250.029.607046
TCAACGC161350.028.9676634
GTACATG242100.026.8444611
TACATGG245050.026.0415362
GTACTAG31850.025.3957161
ACGCAGA181450.025.377647
CGCAGAG188450.024.159878
ACATGGG257400.024.0234813
CATGGGG203900.021.9771674
GCATCGT33400.021.9454828
GTATAAG34150.020.931211
ATGGGGG137550.020.8842985
GCAGAGT214500.020.7052829
TAGGCAT45200.020.2831545
TGATCGC20900.019.7885079
Produced by FastQC (version 0.11.5)