FastQCFastQC Report
Thu 26 May 2016
ERR580038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5745402
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80550.043.99141
GTATCAA132650.038.024531
CAACGCA149550.032.680935
ATCAACG153400.031.6771763
TATCAAC165200.030.4096452
AACGCAG167550.029.7018786
TCAACGC169200.029.1355084
GTACTAG31450.025.9548721
ACGCAGA189600.025.5040667
GTACATG244200.024.7319151
CGCAGAG198800.024.1583278
TACATGG249300.023.9023552
ACATGGG254550.022.3943753
GTGGTAT38200.021.3686051
GCAGAGT221550.021.2109749
TAGGCAT43500.020.6343865
TAGTACT44300.020.1556764
CATGGGG204100.020.1010484
CTAGTAC45600.020.0965273
CGTTAAC9750.019.357481
Produced by FastQC (version 0.11.5)