FastQCFastQC Report
Thu 26 May 2016
ERR580037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6575302
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197530.3004120571192015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157220.23910688817030762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94520.14375005132844088No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114650.050.776231
GTATCAA210800.040.9335441
CAACGCA243050.034.2317775
GTACTAG55650.033.630381
ATCAACG248450.033.4854623
TATCAAC266550.032.04752
AACGCAG274400.030.817846
TAGGCAT68900.030.359365
TCAACGC286350.029.2850634
GCATCGT57550.029.2247968
TAGTACT68300.028.6299864
GGGCACC60050.028.49399894
ACGCAGA291050.028.4582657
CTGTGCG68600.028.2865129
CTAGGCA75000.028.2659654
CGCAGAG296700.027.8840358
GTGGTAT34850.027.7957441
GTACATG301900.027.3355541
TACATGG306000.026.7789752
ACATGGG299750.026.3434183
Produced by FastQC (version 0.11.5)