FastQCFastQC Report
Thu 26 May 2016
ERR580037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6575302
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174440.26529579934123176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144410.219624893274864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92170.14017607100023696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113250.049.8581431
GTATCAA206700.042.2151951
CAACGCA245350.034.0587235
ATCAACG249250.033.5828863
GTACTAG53250.032.5790251
TATCAAC269850.032.1436582
AACGCAG279000.030.5728036
TAGGCAT63700.029.5860925
TCAACGC287150.029.3465754
GTGGTAT35300.028.1021161
ACGCAGA296900.028.0805917
GGGCACC60150.027.81572794
CGCAGAG302650.027.5007158
CTAGGCA71450.027.4296194
GTACATG296500.026.5913051
TAGTACT73100.026.424724
CTGTGCG70350.025.9861359
ACATGGG296600.025.7655033
TACATGG303800.025.6886732
GCAGAGT325400.025.0719649
Produced by FastQC (version 0.11.5)