FastQCFastQC Report
Thu 26 May 2016
ERR580036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4902785
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53860.10985592882412751No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65850.044.487761
GTATCAA109750.039.0321161
CAACGCA122200.033.7034725
ATCAACG124300.033.1756133
TATCAAC134850.031.697222
AACGCAG140350.030.0678066
TCAACGC140300.029.458654
GTACTAG27700.029.0284981
ACGCAGA156600.026.0169457
CGCAGAG161300.025.17128
GCATCGT27400.023.839048
GTACATG192250.023.1139261
GCAGAGT179700.022.0485179
TACATGG199050.021.8990762
CTAGGCA41700.021.8749624
TGATCGC17450.021.816729
TAGGCAT40650.021.3970015
ACATGGG198650.021.1138173
GTGATCG18150.020.4538488
GTATAAG30150.020.1191981
Produced by FastQC (version 0.11.5)