FastQCFastQC Report
Thu 26 May 2016
ERR580036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4902785
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62650.040.344281
GTATCAA107450.038.2691081
CAACGCA120400.032.2798085
ATCAACG124400.031.3555263
TATCAAC134350.030.257932
AACGCAG137200.029.011356
TCAACGC137800.028.510774
ACGCAGA156850.024.6577787
CGCAGAG161200.023.7008618
GTGGTAT32550.022.7450521
GTACATG194800.022.5855661
GTACTAG27900.022.1414151
TACATGG197350.021.646492
GTGATCG15350.020.8180078
TACCCCG5000.020.6778135
ACATGGG199900.020.6648583
GCAGAGT184400.020.4641289
GTATAAG28850.020.268151
TGGTATC35000.020.006862
TGATCGC16000.019.9722779
Produced by FastQC (version 0.11.5)