FastQCFastQC Report
Thu 26 May 2016
ERR580035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5632656
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151350.2687009467647234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126680.22490278121014315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73580.1306310912649379No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83150.047.738821
GTATCAA138200.037.979361
GTACTAG32950.032.829471
CAACGCA161850.031.2651235
ATCAACG165750.030.5035443
TATCAAC177950.028.9680652
AACGCAG180700.028.6640226
GTACATG194150.028.02771
TCAACGC181850.027.9314
GTGGTAT30350.027.4287531
TACATGG197050.026.9956072
TAGTACT49350.026.7790534
CTGTGCG49850.026.7758759
ACATGGG196900.026.0838853
CTAGTAC51500.025.7535573
ACGCAGA199450.025.6149647
GCATCGT37100.025.4622768
CGCAGAG206450.024.6540728
CATGGGG160050.024.3010984
TAGGCAT46550.024.2466745
Produced by FastQC (version 0.11.5)