FastQCFastQC Report
Thu 26 May 2016
ERR580035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5632656
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132170.23464951525532535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112910.2004560548345221No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71160.1263347166949304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79750.047.9810561
GTATCAA138700.040.3317031
CAACGCA161700.033.281745
ATCAACG164500.032.6009523
TATCAAC177600.031.388572
GTACTAG34050.031.2009221
AACGCAG179950.030.5577556
TCAACGC183950.029.5371514
GTACATG183650.028.7195761
TACATGG188200.027.722552
ACGCAGA196300.027.437717
CGCAGAG204250.026.3242028
ACATGGG189750.026.1077443
GTGGTAT29550.025.1348131
CTAACGC15450.024.6414953
CTGTGCG49850.024.6074499
CATGGGG155800.024.254884
TAGTACT48600.024.1776624
TCTAACG16400.024.0749422
TAGGCAT46400.023.905875
Produced by FastQC (version 0.11.5)