FastQCFastQC Report
Thu 26 May 2016
ERR580034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6683376
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197450.2954345229117739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163360.24442736724673278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88850.1329417946857995No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105250.052.095151
GTATCAA190550.041.0396961
CAACGCA224650.033.7305375
ATCAACG227450.033.334723
TATCAAC240350.032.2737962
AACGCAG245900.031.2200646
TCAACGC246650.030.8748664
GTGGTAT39250.029.4724541
ACGCAGA269800.028.0195317
GTACTAG34200.027.22451
CGCAGAG277300.027.1596368
GTACATG235950.025.3904441
TACATGG238100.024.7401262
GCAGAGT305000.024.2803659
ACATGGG244000.023.2908253
TGGTATC48500.023.1668192
CTGTGCG44150.022.0312169
GCATCGT34150.021.8749318
GAGTACT177350.021.85978712-13
TAGTACT45800.021.6564944
Produced by FastQC (version 0.11.5)