FastQCFastQC Report
Thu 26 May 2016
ERR580034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6683376
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171250.2562327781648077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152150.22765440699430944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85830.1284231202913019No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105000.051.673481
GTATCAA191750.040.1388131
CAACGCA226100.033.0317465
ATCAACG227700.032.7797363
TATCAAC241650.031.733532
TCAACGC243150.030.9670414
AACGCAG246500.030.9431366
GTACTAG31800.029.2744881
GTGGTAT38550.027.6855221
ACGCAGA271250.027.4093957
CGCAGAG276850.026.7017948
GTACATG232100.024.065371
GCAGAGT311600.023.4970479
TACATGG236200.023.2495292
CTAACGC15600.023.1997933
ACATGGG233900.022.4260063
TAGTACT48250.022.112614
CTGTGCG45550.021.5626019
TCTAACG17050.021.5090622
GAGTACT182550.021.44435312-13
Produced by FastQC (version 0.11.5)