##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580034_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6683376
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52747997419269	34.0	31.0	34.0	31.0	34.0
2	32.64495279032633	34.0	31.0	34.0	31.0	34.0
3	32.69804272571228	34.0	31.0	34.0	31.0	34.0
4	36.21818524051318	37.0	35.0	37.0	35.0	37.0
5	36.16076186645791	37.0	35.0	37.0	35.0	37.0
6	36.1975037765345	37.0	37.0	37.0	35.0	37.0
7	36.14412925443668	37.0	36.0	37.0	35.0	37.0
8	36.136587856197224	37.0	36.0	37.0	35.0	37.0
9	37.85642046773966	39.0	38.0	39.0	35.0	39.0
10-11	37.80905391227428	39.0	38.0	39.0	35.0	39.0
12-13	37.77913617309575	39.0	38.0	39.0	35.0	39.0
14-15	39.16984844186531	40.0	39.0	41.0	36.0	41.0
16-17	39.16073126814951	40.0	39.0	41.0	36.0	41.0
18-19	39.15209274474457	40.0	39.0	41.0	36.0	41.0
20-21	39.123899583084956	40.0	39.0	41.0	36.0	41.0
22-23	39.0474522157664	40.0	39.0	41.0	35.0	41.0
24-25	38.98305265183345	40.0	38.0	41.0	35.0	41.0
26-27	38.865015076212984	40.0	38.0	41.0	35.0	41.0
28-29	38.76108212376499	40.0	38.0	41.0	35.0	41.0
30-31	38.608544469142544	40.0	38.0	41.0	34.5	41.0
32-33	38.50137557725317	40.0	38.0	41.0	34.0	41.0
34-35	38.405607285898625	40.0	38.0	41.0	34.0	41.0
36-37	38.2819551825305	40.0	38.0	41.0	34.0	41.0
38-39	38.16788887831539	40.0	38.0	41.0	34.0	41.0
40-41	38.054689127171656	40.0	37.5	41.0	33.0	41.0
42-43	38.05633754557577	40.0	38.0	41.0	33.0	41.0
44-45	38.022694443646444	40.0	37.5	41.0	33.0	41.0
46-47	37.84421540850013	40.0	37.0	41.0	33.0	41.0
48-49	37.65206543818573	40.0	37.0	41.0	33.0	41.0
50-51	37.46793716229642	40.0	36.5	41.0	32.5	41.0
52-53	37.306866694317364	40.0	36.0	41.0	32.0	41.0
54-55	37.100909704915594	39.0	36.0	41.0	32.0	41.0
56-57	36.875131146294926	39.0	35.0	41.0	31.0	41.0
58-59	36.64368157649667	39.0	35.0	41.0	31.0	41.0
60-61	36.39286372635626	38.5	35.0	40.0	31.0	41.0
62-63	36.10018574445011	38.0	35.0	40.0	30.5	41.0
64-65	35.80211572714149	37.0	35.0	40.0	30.0	41.0
66-67	35.481654556020786	37.0	34.5	40.0	29.5	41.0
68-69	35.158924322079145	36.0	34.0	39.0	29.0	41.0
70-71	34.829501811659256	36.0	34.0	39.0	29.0	41.0
72-73	34.50670417166414	35.0	34.0	38.5	29.0	40.0
74-75	34.148077408782626	35.0	34.0	37.5	29.0	39.5
76-77	32.612280829329364	34.5	31.5	36.0	26.5	39.0
78-79	33.49166805817898	35.0	33.0	36.0	28.0	39.0
80-81	33.46431908065624	35.0	34.0	36.0	29.0	38.5
82-83	33.23360858344645	35.0	33.0	36.0	28.5	37.0
84-85	33.01348270694332	35.0	33.0	35.0	28.0	37.0
86-87	32.80893458635276	35.0	33.0	35.0	27.0	36.5
88-89	32.62463850604844	35.0	33.0	35.0	27.0	36.0
90-91	32.46036247249893	35.0	33.0	35.0	27.0	36.0
92-93	32.30699634436249	35.0	33.0	35.0	26.0	36.0
94-95	32.17307323424569	35.0	33.0	35.0	26.0	35.0
96-97	32.05166804920148	35.0	32.5	35.0	25.0	35.0
98-99	31.95045453076409	35.0	32.0	35.0	25.0	35.0
100	30.0962477646028	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.028745731381199846
1101	2	-0.046449720096660485
1101	3	-0.037115395278213725
1101	4	-0.020211392961869024
1101	5	-0.013203849444522575
1101	6	-0.04952570687463265
1101	7	-0.05634564305743339
1101	8	0.023364334075033355
1101	9	-0.041654382040746896
1101	10-11	0.024300456895055333
1101	12-13	-0.005613337773446858
1101	14-15	-0.0494130939570141
1101	16-17	-0.03206117630775651
1101	18-19	-0.02691470057978762
1101	20-21	-0.023278542402707103
1101	22-23	-0.10148233015824815
1101	24-25	-0.04979030061385714
1101	26-27	-0.09044890261427696
1101	28-29	-0.043227475423258
1101	30-31	-0.11554346062177956
1101	32-33	-0.10380133369092448
1101	34-35	-0.2076862475362944
1101	36-37	-0.10051199238554176
1101	38-39	-0.132844963919716
1101	40-41	-0.14553663350599066
1101	42-43	-0.08077337553717712
1101	44-45	-0.05913172326272331
1101	46-47	-0.10107138520346126
1101	48-49	-0.05188518589501001
1101	50-51	0.03240348042429275
1101	52-53	0.008312929975048178
1101	54-55	-0.04328532006012864
1101	56-57	-0.024415379817014582
1101	58-59	-0.056499022141196065
1101	60-61	-0.06129773507585412
1101	62-63	-0.0839742287137355
1101	64-65	-0.01110333530751717
1101	66-67	-0.018068236490385914
1101	68-69	-0.08215802451244514
1101	70-71	-0.06252783619569868
1101	72-73	-0.07674118577877209
1101	74-75	-0.08905216764273405
1101	76-77	-0.15002753429372007
1101	78-79	-0.07896811993112607
1101	80-81	-5.717379866183592E-4
1101	82-83	-0.032405993894847995
1101	84-85	-0.08103823375170549
1101	86-87	-0.16645624122431713
1101	88-89	-0.10335408701161697
1101	90-91	-0.07729324988611808
1101	92-93	-0.060189196039502235
1101	94-95	-0.1032601002529887
1101	96-97	-0.16182210469945346
1101	98-99	-0.21296297881442783
1101	100	-0.33317976833454566
1102	1	-0.08350309474396767
1102	2	-0.08906600285403954
1102	3	-0.09020077384606395
1102	4	-0.07341383385853817
1102	5	-0.050144972688201506
1102	6	-0.026599143830878802
1102	7	-0.02381755738343827
1102	8	-0.011276348002347447
1102	9	-0.10517350137703829
1102	10-11	-0.04332280160986812
1102	12-13	-0.03973486023969741
1102	14-15	-0.0639085888419828
1102	16-17	-0.06051686035426229
1102	18-19	-0.05551032161907443
1102	20-21	-0.0871966479705506
1102	22-23	-0.12175949584148071
1102	24-25	-0.10633433560810346
1102	26-27	-0.12252051996757274
1102	28-29	-0.10240452049900028
1102	30-31	-0.11062384028573291
1102	32-33	-0.140147221279733
1102	34-35	-0.1163882423951037
1102	36-37	-0.13123940669960632
1102	38-39	-0.23303063818209324
1102	40-41	-0.224594510867405
1102	42-43	-0.09878134320346987
1102	44-45	-0.10372365589810784
1102	46-47	-0.07149714576493693
1102	48-49	-0.025257376815133625
1102	50-51	-0.07202258014858387
1102	52-53	-0.13661087219202983
1102	54-55	-0.13384098990191262
1102	56-57	-0.09887810255558804
1102	58-59	-0.18231722246343907
1102	60-61	-0.26681986224355114
1102	62-63	-0.3015417053549285
1102	64-65	-0.2608985365237757
1102	66-67	-0.2587653265003169
1102	68-69	-0.23781569943850656
1102	70-71	-0.2390094017729112
1102	72-73	-0.2705307808333117
1102	74-75	-0.24230163605098198
1102	76-77	-0.2489040591004894
1102	78-79	-0.16995112515311206
1102	80-81	-0.23180983490139795
1102	82-83	-0.16213652143017754
1102	84-85	-0.17516995998213503
1102	86-87	-0.22099643395029744
1102	88-89	-0.15429255115096652
1102	90-91	-0.15823562816143522
1102	92-93	-0.17627353349060115
1102	94-95	-0.23809282339431093
1102	96-97	-0.2883418590277351
1102	98-99	-0.3240402006412708
1102	100	-0.13987805356677185
1103	1	0.03146387201960721
1103	2	4.084106071857718E-4
1103	3	0.011341580074009983
1103	4	0.05011711540910113
1103	5	0.005860284393442328
1103	6	0.030502149429331382
1103	7	0.039173460695593576
1103	8	0.02389215453306548
1103	9	-0.0391006133852585
1103	10-11	0.014206827902285113
1103	12-13	0.021976419520086665
1103	14-15	0.055472416266042046
1103	16-17	0.07347135056145504
1103	18-19	0.12077266600780234
1103	20-21	0.06593767590278787
1103	22-23	0.014811837133017036
1103	24-25	-0.015148473065387691
1103	26-27	-0.0018729526317784462
1103	28-29	0.05888493354815694
1103	30-31	0.04285663918567906
1103	32-33	-0.015470524407824371
1103	34-35	0.004239471372969206
1103	36-37	0.05802948358254412
1103	38-39	0.06617459905790923
1103	40-41	-0.021739806311146026
1103	42-43	0.029364386037748602
1103	44-45	0.0818400459134736
1103	46-47	0.11503161829860176
1103	48-49	0.03594627166248898
1103	50-51	0.07464452452872194
1103	52-53	0.08896247857269657
1103	54-55	0.14473493574534047
1103	56-57	0.16806203458546776
1103	58-59	0.16991856351486234
1103	60-61	0.02891396973826943
1103	62-63	0.10049345823488665
1103	64-65	0.11456860659672685
1103	66-67	0.08660730171379072
1103	68-69	0.1129246538142823
1103	70-71	0.06760208668184475
1103	72-73	0.006286965322907179
1103	74-75	0.027370435476235855
1103	76-77	-0.03125312846301398
1103	78-79	-0.025734952353381857
1103	80-81	-0.02723392737871677
1103	82-83	0.06479767514184687
1103	84-85	0.08332789165912402
1103	86-87	0.03746603899102041
1103	88-89	0.04094646791823209
1103	90-91	0.03852454384081483
1103	92-93	0.058888279644527586
1103	94-95	0.038549984808774695
1103	96-97	-0.0357894056633441
1103	98-99	-6.303941013641179E-7
1103	100	0.1556943027309572
1104	1	0.013356282123403673
1104	2	-0.02431462802717732
1104	3	0.007910574761048395
1104	4	-0.0025411729620472556
1104	5	-0.014711345064299053
1104	6	-0.044564065166348144
1104	7	-0.021561756681713007
1104	8	-0.02569459972860244
1104	9	-0.0726950948870595
1104	10-11	2.7629339052737123E-4
1104	12-13	-0.04965721044651872
1104	14-15	-0.07909991883956735
1104	16-17	-0.038107332404884176
1104	18-19	-0.05777875508676544
1104	20-21	-0.048432153634315966
1104	22-23	-0.0895663783368974
1104	24-25	-0.07751725723665714
1104	26-27	-0.11062121827161064
1104	28-29	-0.08527152849713104
1104	30-31	-0.16718825440683815
1104	32-33	-0.17682426561674447
1104	34-35	-0.08368212882971449
1104	36-37	-0.0986606196637112
1104	38-39	-0.10700476651242496
1104	40-41	-0.09315632745679636
1104	42-43	-0.11773594480304439
1104	44-45	-0.08656462277123467
1104	46-47	-0.0955605436828435
1104	48-49	-0.08439030841476125
1104	50-51	-0.03519861968126747
1104	52-53	-0.10875370776265214
1104	54-55	-0.05459815206327079
1104	56-57	-0.09440313029772796
1104	58-59	-0.14935370600905173
1104	60-61	-0.12649976323086776
1104	62-63	-0.15030522784953604
1104	64-65	-0.1112387574761371
1104	66-67	-0.03909113904863659
1104	68-69	-0.10186820614944736
1104	70-71	-0.09813141631246225
1104	72-73	0.01850836158637037
1104	74-75	0.05265363631909281
1104	76-77	-0.07154141163938732
1104	78-79	-0.008086637009867559
1104	80-81	0.023539765439011262
1104	82-83	0.03334799920101261
1104	84-85	-0.029656842294230046
1104	86-87	0.03599588427037048
1104	88-89	-0.029945955998684326
1104	90-91	0.015288973474575585
1104	92-93	0.07822652215233461
1104	94-95	-0.030211340596672187
1104	96-97	-0.08022063571063143
1104	98-99	-0.09380996818780218
1104	100	0.0189874511909629
1105	1	0.028754123614525895
1105	2	-0.003866680392057731
1105	3	-0.011373650559384885
1105	4	0.010443952136718337
1105	5	-0.01732010358919922
1105	6	-0.006098829752360757
1105	7	0.016385191336624416
1105	8	0.010384454428340462
1105	9	-0.031551354082083094
1105	10-11	-0.006545355511534012
1105	12-13	0.022916223409829684
1105	14-15	0.03578581527050062
1105	16-17	-0.014646541280555425
1105	18-19	-0.03855753919572891
1105	20-21	-0.04505227532417422
1105	22-23	-0.012732281646222532
1105	24-25	-0.044581338229448875
1105	26-27	-0.0696276244107068
1105	28-29	-0.08111553202989086
1105	30-31	-0.038210129147771
1105	32-33	-0.06485118873628437
1105	34-35	0.01470391072233923
1105	36-37	-0.03596065167862861
1105	38-39	-0.06500856145138556
1105	40-41	-0.048424872815651554
1105	42-43	-0.0766685846479902
1105	44-45	-0.10721025946786966
1105	46-47	-0.08598962902893703
1105	48-49	-0.1274307931305998
1105	50-51	-0.06069715717379154
1105	52-53	-0.09214058241133927
1105	54-55	-0.02403924679239111
1105	56-57	-0.06275591593527707
1105	58-59	-0.036925927011623116
1105	60-61	0.02607901542825175
1105	62-63	0.03529931382314544
1105	64-65	0.06215522246177585
1105	66-67	-0.05225723054551423
1105	68-69	-0.08152051387584436
1105	70-71	0.05080960720712824
1105	72-73	-0.007963614581200318
1105	74-75	-0.04455221162855594
1105	76-77	-0.028480438205782832
1105	78-79	0.027767398278221833
1105	80-81	0.009041147166357177
1105	82-83	0.07960079008339704
1105	84-85	0.07391717323900338
1105	86-87	0.01348474924539289
1105	88-89	0.046562935563521535
1105	90-91	0.0011332389035985102
1105	92-93	0.02748051745805924
1105	94-95	0.02910206922648939
1105	96-97	0.05520268273514972
1105	98-99	0.08127019060932028
1105	100	0.08178740964688913
1106	1	-0.01262953676799583
1106	2	-0.01374983897277815
1106	3	-0.03351230714247322
1106	4	-0.017945431695366665
1106	5	-0.029620722070120564
1106	6	-0.0151617712013703
1106	7	-0.027201811766069284
1106	8	-0.010953940025835607
1106	9	-0.0646591870148967
1106	10-11	-0.05166663226276569
1106	12-13	-0.04132415215929086
1106	14-15	0.01085575312086462
1106	16-17	0.011440511931361641
1106	18-19	-0.004183406129733669
1106	20-21	-0.011508824680255714
1106	22-23	-0.05699894105813996
1106	24-25	-0.07579224859618705
1106	26-27	-0.0890348623828956
1106	28-29	0.038736997395311334
1106	30-31	-0.01076192302271295
1106	32-33	0.02453648136015829
1106	34-35	-0.030332845505931516
1106	36-37	-0.039836711422317705
1106	38-39	0.06888040563154618
1106	40-41	0.13070362658233137
1106	42-43	0.07585894261320192
1106	44-45	0.12003799974637275
1106	46-47	0.13980566208706335
1106	48-49	0.15841753793939262
1106	50-51	0.113198076223469
1106	52-53	0.1614728805816128
1106	54-55	0.22130625989085217
1106	56-57	0.12255474310485681
1106	58-59	0.07244145682896885
1106	60-61	0.09804414995868171
1106	62-63	0.12732419274505702
1106	64-65	0.1017887071515915
1106	66-67	0.08860706319638467
1106	68-69	0.15759187030531763
1106	70-71	0.15983147017426802
1106	72-73	0.10311733860808658
1106	74-75	0.04971195119078686
1106	76-77	0.01752760561149813
1106	78-79	0.12918954254880788
1106	80-81	0.13391811348798655
1106	82-83	0.04735116639040626
1106	84-85	0.12980136921380137
1106	86-87	0.1468974236698486
1106	88-89	0.05861570182357667
1106	90-91	0.09701771469188003
1106	92-93	0.11296749446847087
1106	94-95	0.131828849407853
1106	96-97	0.1246308823291713
1106	98-99	0.10868856400520954
1106	100	0.15860266303817738
1107	1	0.01802091728271904
1107	2	0.03619015566633266
1107	3	0.026335679276613178
1107	4	0.04260363258651978
1107	5	0.039307263374261936
1107	6	0.035920965124311977
1107	7	0.008392231153500518
1107	8	0.030540630047433126
1107	9	0.02534682367438279
1107	10-11	0.028856297704578537
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2316	36-37	-0.1969983834783804
2316	38-39	-0.1844026109723771
2316	40-41	-0.14705231649647033
2316	42-43	-0.2096594714300224
2316	44-45	-0.22963050734173862
2316	46-47	-0.2173934408815299
2316	48-49	-0.1855181320821302
2316	50-51	-0.19386971140483666
2316	52-53	-0.2247362955615415
2316	54-55	-0.19182325720942117
2316	56-57	-0.35926852524448094
2316	58-59	-0.36008915952793075
2316	60-61	-0.22631353517282093
2316	62-63	-0.15233959043085576
2316	64-65	-0.1229470342917196
2316	66-67	-0.17468703320336942
2316	68-69	-0.11196448123729397
2316	70-71	-0.1228851233505992
2316	72-73	-0.0648225626627692
2316	74-75	-0.024376325562073475
2316	76-77	-0.1422013294667508
2316	78-79	-0.12274949338814167
2316	80-81	-0.15162264033946826
2316	82-83	-0.18007775787629754
2316	84-85	-0.08015790521621824
2316	86-87	-0.18805838000542252
2316	88-89	-0.2199702840263953
2316	90-91	-0.22834518906234536
2316	92-93	-0.1627338334689128
2316	94-95	-0.17320304877350878
2316	96-97	-0.1364362590630286
2316	98-99	-0.13571688649262015
2316	100	-0.2907957599491553
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	38.0
14	259.0
15	843.0
16	2326.0
17	5276.0
18	9664.0
19	15079.0
20	22397.0
21	32594.0
22	42099.0
23	43419.0
24	40768.0
25	40153.0
26	45505.0
27	55450.0
28	68373.0
29	85218.0
30	105194.0
31	132722.0
32	169296.0
33	224066.0
34	328101.0
35	495463.0
36	872799.0
37	1624412.0
38	1796838.0
39	424864.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.27969034488455	20.153319263904734	13.569152877549287	23.997837513661434
2	15.603183459945724	20.859867654181443	39.61738492182042	23.91956396405242
3	18.314905793460532	26.166528062359667	30.052306542252964	25.466259601926833
4	12.631123766231726	17.390190602375927	38.34714515391757	31.631540477474772
5	13.258906105904675	37.83281975573628	35.04702104293986	13.86125309541919
6	30.679458016022153	37.661293978742165	17.78190975781533	13.87733824742035
7	26.728122252016178	32.52333537174474	22.79789846616399	17.950643910075083
8	25.69269630716094	35.23071621376745	20.244662988726315	18.831924490345294
9	26.06115695925438	15.460278201096914	20.81260815211658	37.66595668753212
10-11	24.860926968011146	26.062553275979937	28.33929016831207	20.737229587696845
12-13	25.48874662202652	23.911503640902172	28.347895096651076	22.251854640420234
14-15	22.868835104787227	25.31092775206279	25.99993566057512	25.820301482574866
16-17	22.11740901410381	28.03115373963229	26.87078122701852	22.980656019245377
18-19	21.778161486659112	27.427864122666808	28.967154361818604	21.826820028855472
20-21	22.87643383102443	26.62090377411071	28.709261243872376	21.793401150992484
22-23	22.88414483515323	26.407601252501212	28.394286806138375	22.313967106207187
24-25	22.501615780038154	26.540837694885816	28.724046614964198	22.23349991011183
26-27	22.485999288966777	26.730749433806878	28.3695747805852	22.41367649664115
28-29	22.091273510057736	26.897192326677455	28.64474816046951	22.3667860027953
30-31	22.58830755557085	26.607521268664673	28.570653712884344	22.233517462880133
32-33	22.647818491084596	26.86069261965411	28.141608651102217	22.349880238159077
34-35	22.7500655362119	26.86152767004702	28.42128203194576	21.96712476179532
36-37	22.633467884235998	26.6368142372327	28.259101151689194	22.470616726842117
38-39	22.57841837923691	26.688444822002293	28.51195198780842	22.22118481095237
40-41	22.55881741435529	26.29141620038609	28.738372662403037	22.41139372285558
42-43	22.391761959355954	26.480496907570057	28.3824527980603	22.74528833501369
44-45	22.48429886832746	26.919953668325725	28.19462834482772	22.401119118519098
46-47	22.529794074941258	26.731062388002886	27.818130155458277	22.92101338159758
48-49	22.79429149293522	26.68179821174826	27.85900458615104	22.664905709165478
50-51	22.69574145429453	26.803910904893357	27.62329277985235	22.87705486095976
52-53	22.417940762868625	27.03297512299047	27.559397387834693	22.98968672630621
54-55	22.329825352092445	26.988195203205244	27.561953037581965	23.120026407120346
56-57	22.73466143207974	26.811047995974928	27.3685427261624	23.085747845782933
58-59	22.33634561634339	26.933507567218236	27.680585476130304	23.049561340308074
60-61	22.440859311596935	27.14226917416307	27.39757328293341	23.019298231306585
62-63	22.4807818486384	26.866859639974354	27.32227465104326	23.330083860343986
64-65	22.109676224244158	26.823939451252283	27.44549571279625	23.620888611707304
66-67	22.36091388863535	26.674689402656554	27.38192213482087	23.58247457388723
68-69	22.144797875696717	26.708274813071203	27.475277098569084	23.671650212663
70-71	22.130917270491597	26.432909464453154	27.510775404956295	23.925397860098954
72-73	22.218956021736886	26.558828680818202	27.507043508558716	23.715171788886202
74-75	22.258518375254805	26.735654380085087	27.320862822762653	23.68496442189745
76-77	22.079861943956107	26.652579355964907	27.216480776910895	24.051077923168094
78-79	21.9095623031297	26.642073068408873	27.287178078744155	24.16118654971727
80-81	21.790185816079422	26.871266428053232	27.310293004340963	24.028254751526383
82-83	22.056057313925237	26.41329494169805	27.75128373215056	23.779364012226146
84-85	22.127414247316395	26.506129196921872	27.235665640938013	24.130790914823724
86-87	21.498919852089877	26.810052950627423	27.486421932894288	24.204605264388412
88-89	21.790285948813832	26.281722776179357	27.43688811369304	24.49110316131377
90-91	21.72111395453681	26.254333032132703	27.45257436760212	24.57197864572836
92-93	21.754863846706023	26.25762387000266	27.42223138260764	24.565280900683675
94-95	21.238468511146273	26.160720083815857	27.710578819617243	24.890232585420623
96-97	21.284370367133963	26.124800769927347	27.59031264532648	25.000516217612205
98-99	21.039740013749245	26.309340934794484	27.476650113159316	25.174268938296958
100	21.003574243660236	26.175080483324887	27.236401026308275	25.584944246706602
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	576.0
1	410.0
2	371.5
3	511.0
4	449.0
5	433.5
6	693.5
7	1344.5
8	2201.5
9	2844.0
10	2905.5
11	2765.5
12	2997.0
13	3439.0
14	3891.0
15	4532.0
16	5244.5
17	5897.0
18	6685.0
19	7478.5
20	8502.5
21	9648.5
22	11042.5
23	13048.5
24	15751.5
25	19203.0
26	23677.5
27	29371.5
28	36690.0
29	46934.5
30	60083.0
31	73981.0
32	90835.0
33	108608.5
34	125307.5
35	142022.5
36	160771.5
37	184453.0
38	198314.5
39	201261.5
40	201193.5
41	201245.5
42	207750.5
43	219178.5
44	232326.5
45	252847.0
46	270389.0
47	281586.5
48	290914.0
49	290334.0
50	282723.0
51	278384.5
52	271707.0
53	259390.5
54	247632.0
55	224839.0
56	199328.5
57	170422.5
58	142432.0
59	126369.0
60	104997.5
61	80853.5
62	62957.0
63	49013.0
64	35643.0
65	23564.0
66	16206.0
67	11743.5
68	8840.5
69	6701.5
70	5037.0
71	3644.0
72	2648.5
73	1946.0
74	1395.0
75	947.0
76	573.5
77	333.5
78	202.0
79	107.5
80	58.5
81	32.5
82	19.0
83	10.0
84	7.0
85	4.5
86	3.0
87	1.0
88	1.0
89	1.5
90	1.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004907699342368288
2	0.03130154580559286
3	0.001226924835592072
4	0.0013615873175472995
5	0.007735611463428063
6	7.481248997512635E-5
7	0.001002487365666693
8	0.0070174115596668505
9	0.0012119623375970467
10-11	2.5436246591542956E-4
12-13	7.481248997512635E-5
14-15	0.0010623373576467941
16-17	7.481248997512635E-5
18-19	0.0010249311126592309
20-21	2.0199372293284114E-4
22-23	0.0016309122814577542
24-25	0.0010698186066443068
26-27	0.002154599711283639
28-29	1.945124739353285E-4
30-31	0.0019226809923607471
32-33	0.0016757997754428301
34-35	7.181999037612129E-4
36-37	0.0041820181896095624
38-39	0.0010997436026343572
40-41	0.003852843233719007
42-43	0.004219424434597125
44-45	0.0038752869807115444
46-47	0.002513699663164245
48-49	0.009695698700776374
50-51	0.00706978030264944
52-53	0.005057324322318541
54-55	0.004937624338358339
56-57	0.0039426182216891585
58-59	0.006059811687985234
60-61	0.008401442624206688
62-63	0.00360596201680109
64-65	0.0034712995348458626
66-67	0.004279274426577227
68-69	0.002236893450256278
70-71	0.0032393808159229707
72-73	0.006725642848763858
74-75	0.008132117660296234
76-77	0.004384011912542404
78-79	0.0029999808480025664
80-81	0.002715693386097086
82-83	0.004832886852393162
84-85	0.0023865184302065304
86-87	0.0039426182216891585
88-89	7.032374057661877E-4
90-91	0.001840387253388108
92-93	1.2718123295771478E-4
94-95	0.0023042246912338914
96-97	0.00221444970326374
98-99	0.0014887685505050143
100	5.984999198010108E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6683376.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.638354900896054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.53921171236094	45.94474385559531
2	15.4880805053888	19.093196058496083
3	4.795621147829308	8.867825948404361
4	2.000609492418005	4.932571116470499
5	0.9727982902197593	2.9980843129775194
6	0.5702389441016112	2.10891542489084
7	0.35994251082917833	1.5530384958485959
8	0.2475577018538876	1.220723958825611
9	0.17743654058051295	0.9843206814620012
>10	0.8030286099401096	8.424132438253851
>50	0.032405079895088416	1.3645316895024373
>100	0.012180927861654062	1.273206745423701
>500	5.821465789400164E-4	0.2500064504936546
>1k	2.1447309948850282E-4	0.21185624124922656
>5k	3.063901419834063E-5	0.16209325353023343
>10k+	6.127802839668127E-5	0.6107533285759932
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17125	0.2562327781648077	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15215	0.22765440699430944	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8583	0.1284231202913019	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.496249799502527E-5	0.0
9	0.0	0.0	0.0	1.496249799502527E-5	4.4887493985075805E-5
10-11	0.0	0.0	0.0	1.496249799502527E-5	4.4887493985075805E-5
12-13	0.0	0.0	0.0	2.2443746992537902E-5	4.4887493985075805E-5
14-15	0.0	0.0	0.0	2.992499599005054E-5	4.4887493985075805E-5
16-17	0.0	0.0	0.0	4.4887493985075805E-5	5.984999198010108E-5
18-19	0.0	0.0	0.0	4.4887493985075805E-5	8.229373897263897E-5
20-21	0.0	0.0	0.0	7.481248997512634E-5	8.977498797015161E-5
22-23	0.0	7.481248997512635E-6	0.0	2.618437149129422E-4	1.1969998396020214E-4
24-25	0.0	1.496249799502527E-5	0.0	6.95756156768675E-4	1.6458747794527795E-4
26-27	0.0	1.496249799502527E-5	0.0	0.00181794350639557	1.6458747794527795E-4
28-29	0.0	1.496249799502527E-5	0.0	0.0051770243062787425	1.6458747794527795E-4
30-31	0.0	1.496249799502527E-5	0.0	0.018748009987766663	1.8703122493781587E-4
32-33	0.0	1.496249799502527E-5	0.0	0.051553286841859565	2.768062129079675E-4
34-35	0.0	1.496249799502527E-5	0.0	0.10752799184124909	2.842874619054801E-4
36-37	0.0	1.496249799502527E-5	0.0	0.19681669862656237	2.842874619054801E-4
38-39	0.0	1.496249799502527E-5	0.0	0.3189331260129611	2.9924995990050535E-4
40-41	0.0	1.496249799502527E-5	0.0	0.4451118716050092	3.06731208898018E-4
42-43	0.0	1.496249799502527E-5	0.0	0.5760636540574704	3.216937068930433E-4
44-45	0.0	1.496249799502527E-5	0.0	0.7391099947092608	3.516187028830938E-4
46-47	0.0	1.496249799502527E-5	0.0	0.937063843183445	4.0398744586568223E-4
48-49	0.0	1.496249799502527E-5	0.0	1.1480949149052815	4.937624338358338E-4
50-51	0.0	1.496249799502527E-5	0.0	1.3638466547445485	4.937624338358338E-4
52-53	0.0	1.496249799502527E-5	0.0	1.5730523017109914	5.461311768184224E-4
54-55	0.0	1.496249799502527E-5	0.0	1.806527120425366	5.835374218059855E-4
56-57	0.0	1.496249799502527E-5	0.0	2.08571985176354	5.835374218059855E-4
58-59	0.0	1.496249799502527E-5	0.0	2.3989073785464115	5.91018670803498E-4
60-61	0.0	1.496249799502527E-5	0.0	2.7198604417887005	5.984999198010107E-4
62-63	0.0	1.496249799502527E-5	0.0	3.044950635726615	6.13462417796036E-4
64-65	0.0	1.496249799502527E-5	0.0	3.3828038404542857	6.13462417796036E-4
66-67	0.0	1.496249799502527E-5	0.0	3.763606895676676	6.209436667935486E-4
68-69	0.0	1.496249799502527E-5	0.0	4.185781257855311	6.284249157910613E-4
70-71	0.0	1.496249799502527E-5	0.0	4.63618985375056	6.433874137860866E-4
72-73	0.0	1.496249799502527E-5	0.0	5.090009899188674	7.181999037612129E-4
74-75	0.0	1.496249799502527E-5	0.0	5.538465889095571	7.181999037612129E-4
76-77	0.0	1.496249799502527E-5	0.0	6.029602404533279	7.331624017562382E-4
78-79	0.0	1.496249799502527E-5	0.0	6.565693745197038	7.481248997512635E-4
80-81	0.0	1.496249799502527E-5	0.0	7.1373060561009884	7.78049895741314E-4
82-83	0.0	1.496249799502527E-5	0.0	7.722272396465499	8.753061327089782E-4
84-85	0.0	1.496249799502527E-5	0.0	8.295298663429978	8.827873817064909E-4
86-87	0.0	1.496249799502527E-5	0.0	8.886826059165308	8.827873817064909E-4
88	0.0	1.496249799502527E-5	0.0	9.353685921606086	8.827873817064909E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10500	0.0	51.67348	1
GTATCAA	19175	0.0	40.138813	1
CAACGCA	22610	0.0	33.031746	5
ATCAACG	22770	0.0	32.779736	3
TATCAAC	24165	0.0	31.73353	2
TCAACGC	24315	0.0	30.967041	4
AACGCAG	24650	0.0	30.943136	6
GTACTAG	3180	0.0	29.274488	1
GTGGTAT	3855	0.0	27.685522	1
ACGCAGA	27125	0.0	27.409395	7
CGCAGAG	27685	0.0	26.701794	8
GTACATG	23210	0.0	24.06537	1
GCAGAGT	31160	0.0	23.497047	9
TACATGG	23620	0.0	23.249529	2
CTAACGC	1560	0.0	23.199793	3
ACATGGG	23390	0.0	22.426006	3
TAGTACT	4825	0.0	22.11261	4
CTGTGCG	4555	0.0	21.562601	9
TCTAACG	1705	0.0	21.509062	2
GAGTACT	18255	0.0	21.444353	12-13
>>END_MODULE