FastQCFastQC Report
Thu 26 May 2016
ERR580033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6787466
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127670.18809670648810617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96360.1419675619737911No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93800.048.624361
GTATCAA168200.041.3733521
CAACGCA193750.034.8947875
ATCAACG195950.034.529553
TATCAAC205150.033.5096932
TCAACGC207150.032.8210374
AACGCAG211350.032.4180876
ACGCAGA240450.028.0452427
CGCAGAG247500.027.1126698
GTACATG241900.023.7725411
GCAGAGT275700.023.593499
TACATGG244250.022.9475482
ACATGGG247750.021.8252683
GAGTACT129950.020.92836212-13
GTACTAG26450.020.621361
GTGGTAT45500.019.8415161
CAGAGTA267700.019.8237810-11
CATGGGG195200.019.7998094
AGAGTAC245950.019.4268110-11
GTGTAGC40950.019.1755391
Produced by FastQC (version 0.11.5)