FastQCFastQC Report
Thu 26 May 2016
ERR580033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6787466
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114220.16828076928856808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93380.13757711640839157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96000.046.1851351
GTATCAA159350.039.2201651
CAACGCA187700.032.2488175
ATCAACG189400.032.0347443
TATCAAC200350.030.7526072
TCAACGC200250.030.2748934
AACGCAG204200.030.0566166
ACGCAGA233150.025.9012267
CGCAGAG243750.024.6398938
GTACTAG26650.024.2476121
GTACATG239050.024.072251
TACATGG243900.022.9492552
ACATGGG245650.021.657323
GCAGAGT274600.021.5123259
GAGTACT128200.020.5832712-13
CATGGGG193150.020.0249084
GTGGTAT46950.019.8918511
AGAGTAC245150.018.92975610-11
ATGGGGG131350.018.8199255
CAGAGTA266450.018.75692410-11
Produced by FastQC (version 0.11.5)