FastQCFastQC Report
Thu 26 May 2016
ERR580032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4939541
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50450.10213499594395511No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61900.042.1534041
GTATCAA110950.041.272611
CAACGCA127000.034.312945
ATCAACG132000.032.90773
TATCAAC139550.032.174562
AACGCAG144250.031.0797946
TCAACGC145900.030.0613334
GTACTAG24900.027.5666391
ACGCAGA162100.026.6426897
GTACATG217900.026.6249141
TACATGG223150.025.5987322
CGCAGAG168750.025.5368148
ACATGGG229000.024.1008343
GCAGAGT187800.022.5726689
CATGGGG184950.022.3925444
TAGGCAT37050.020.6814655
GCATCGT26400.020.6488198
TAGTACT38200.020.4280364
GTGGTAT29200.020.2870081
GTATAAG28000.020.1491
Produced by FastQC (version 0.11.5)