FastQCFastQC Report
Thu 26 May 2016
ERR580032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4939541
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65900.043.79981
GTATCAA114150.038.962061
CAACGCA127450.033.4467625
ATCAACG133200.031.9330043
TATCAAC140600.031.3529032
AACGCAG148100.029.2583146
TCAACGC148750.028.9101774
ACGCAGA165000.025.6634067
CGCAGAG172200.024.344748
GTACTAG26750.024.154771
GTACATG212950.023.6758821
TACATGG219300.023.015792
ACATGGG224600.021.9094943
GCAGAGT191650.021.7024069
CATGGGG184950.020.2022344
GTATAAG29550.019.4719051
CTAGTAC39850.019.3424283
ATGGGGG125450.018.91945
TAGTACT38700.018.7023584
CTGTGCG38300.018.5289849
Produced by FastQC (version 0.11.5)