FastQCFastQC Report
Thu 26 May 2016
ERR580031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10403865
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150610.1447635085614817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA262650.039.493821
GGTATCA147300.037.253681
CAACGCA260950.036.8887525
ATCAACG269450.035.7948463
TATCAAC316700.032.4638022
GTACTAG105150.030.900811
GGGCACC108550.030.87434694
AACGCAG334150.030.3709326
ACGCAGA320250.029.9289197
CGCAGAG324850.029.4332248
TATTCTC47400.028.161865
TAGGCAT133350.027.6340395
TAGTACT136950.027.6294274
CTGTGCG138050.027.2983269
TACCCCG7650.027.0341425
GTACATG438650.027.0136641
TACATGG442600.026.4901052
GCAGAGT354400.026.4645739
GTGGTAT37850.026.2130551
GCATCGT115200.026.1026028
Produced by FastQC (version 0.11.5)