FastQCFastQC Report
Thu 26 May 2016
ERR580031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10403865
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129050.1240404407400519No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA254500.038.296731
GGTATCA144950.037.042311
CAACGCA257600.034.8129435
ATCAACG267350.033.6506083
GGGCACC113400.033.23729794
GTACTAG107300.031.2859021
TATCAAC313600.030.8389852
CTGTGCG138100.029.5379289
AACGCAG327150.029.492436
TAGTACT138950.029.4294014
TAGGCAT134550.028.6093775
ACTGTGC157150.027.9022988
ACGCAGA322850.027.6143657
CGCAGAG327650.027.1383678
GTACATG442400.026.7602881
GTACTGT155350.026.7127116
TACATGG448600.026.1803252
ACATGGG442250.025.8373683
TACTGTG179450.025.6393957
CTAGGCA154150.025.5517834
Produced by FastQC (version 0.11.5)