FastQCFastQC Report
Thu 26 May 2016
ERR580030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052095
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50590.16575499779659544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44580.14606360549065478No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43350.049.6755181
GTATCAA75650.037.912891
CAACGCA85100.032.095825
ATCAACG86200.031.9620763
TATCAAC91450.031.0546282
TCAACGC90900.030.2045424
AACGCAG94350.029.5354676
GTGGTAT20500.026.605421
ACGCAGA108500.025.2484257
CGCAGAG111150.024.5611068
GTACATG115400.024.0387691
TGGTATC22200.022.8740962
GTACTAG14750.022.632491
TACATGG119650.022.2814522
GCAGAGT122950.021.9778219
ACATGGG121550.021.544923
CATGGGG91300.020.236954
TGATCGC8400.020.140429
TAACGCA3354.0017767E-1119.6474634
GTGATCG8700.019.4430568
Produced by FastQC (version 0.11.5)