FastQCFastQC Report
Thu 26 May 2016
ERR580030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052095
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44650.14629295614979218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41720.13669299284589767No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.051.7267231
GTATCAA73550.037.4403041
CAACGCA86150.030.8761795
ATCAACG86950.030.8098133
TATCAAC91500.029.8941142
TCAACGC92500.029.0110824
AACGCAG95150.028.4980556
ACGCAGA108700.024.5132967
GTACTAG13900.024.0853391
GTACATG116100.023.6374151
CGCAGAG112300.023.3926948
TACATGG117350.023.349052
GTGGTAT17850.022.7179681
ACATGGG118850.022.184393
CATGGGG89800.020.463034
GCAGAGT126150.020.3773779
GAGTACT56800.019.27835712-13
CAGAGTA121900.018.5623810-11
AGAGTAC115150.018.28332510-11
TAGGCAT20200.017.914385
Produced by FastQC (version 0.11.5)