FastQCFastQC Report
Thu 26 May 2016
ERR580029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357557
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59940.17852265799210557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49130.1463266297489514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55700.051.8343541
GTATCAA99250.041.1712271
ATCAACG113450.034.6808323
CAACGCA114150.034.427495
TATCAAC121400.033.3042832
AACGCAG125200.032.1236086
TCAACGC125450.031.4388244
ACGCAGA137450.028.4748617
CGCAGAG141050.027.5815478
GTGGTAT21750.026.3757691
GCAGAGT153650.024.6495599
GTACATG155950.023.5790311
TACATGG158150.022.8897912
TGGTATC24050.022.6758352
ACATGGG158650.022.0149273
GAGTACT62750.021.75529512-13
GTACTAG16000.021.4539591
CAGAGTA151350.021.11375810-11
CATGGGG127650.020.291114
GTACTTT67850.019.94912114-15
Produced by FastQC (version 0.11.5)