FastQCFastQC Report
Thu 26 May 2016
ERR580029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357557
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54180.1613673274943657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47820.14242498340311124No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59050.048.644261
GTATCAA95000.036.718981
CAACGCA111250.030.3759025
ATCAACG113450.029.8287323
TATCAAC121900.028.5402492
TCAACGC122750.027.8368284
AACGCAG124200.027.6989926
GTGGTAT23100.027.2711321
ACGCAGA139350.024.3162337
GTACTAG17800.024.2984261
CGTTAAC7450.023.9793641
GTACATG158500.023.4319341
CGCAGAG144550.023.4089748
TACATGG158800.023.0630462
ACATGGG159500.021.7766253
TGGTATC28300.021.762792
GCAGAGT158800.021.1295289
CATGGGG127650.020.0301824
GTATATA13450.019.5738411
GAGTACT67300.019.30761512-13
Produced by FastQC (version 0.11.5)