FastQCFastQC Report
Thu 26 May 2016
ERR580028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11309479
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334160.2954689601528063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256420.2267301614866609No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170830.15105028268764636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167350.046.3376351
GTATCAA300300.040.5116541
CAACGCA334400.034.674795
ATCAACG341850.033.9642873
GTACTAG83750.032.3427241
TATCAAC380150.031.6687982
AACGCAG381100.031.3251746
GCATCGT77200.030.1356728
TCAACGC390400.030.0166194
GTACATG395650.028.5258751
ACGCAGA410100.028.2614427
GGGCACC95850.028.19552694
CTGTGCG105850.027.795879
CGCAGAG418350.027.648078
TACATGG402850.027.6313112
ACATGGG392900.027.26523
TAGTACT115700.026.7027234
TAACGCC35850.026.6275044
CTAACGC35600.026.2869433
TAGGCAT107650.025.9453435
Produced by FastQC (version 0.11.5)