FastQCFastQC Report
Thu 26 May 2016
ERR580028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11309479
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290210.25660775354903614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225560.19944331653120362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173530.15343766056774144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160350.045.7052041
GTATCAA292500.041.127561
CAACGCA341750.033.7769135
ATCAACG347800.033.3388983
TATCAAC384800.031.1720182
AACGCAG387000.030.5063086
TCAACGC389700.029.9947684
GTACTAG81750.028.8671861
GTACATG380650.027.7378231
ACGCAGA424700.027.1784767
TACATGG385500.026.6895182
CGCAGAG434500.026.5438448
ACATGGG381300.025.9599133
GGGCACC92850.025.16136294
CTGTGCG105700.024.6328019
TAGTACT111250.024.5879964
TAGGCAT104500.024.5570665
CTAACGC30950.024.2980253
CATGGGG304400.024.1177354
TAACGCC31800.023.6479234
Produced by FastQC (version 0.11.5)