FastQCFastQC Report
Thu 26 May 2016
ERR580027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7420580
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141160.1902277180489935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108560.14629584210398647No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113150.048.369721
GTATCAA209300.042.0544321
CAACGCA235450.035.7617955
ATCAACG244850.034.392353
TATCAAC259950.033.4445342
AACGCAG269450.032.086696
TCAACGC280900.030.1450944
ACGCAGA289100.028.8663487
CGCAGAG294350.028.2399378
GTACTAG54150.027.4387171
GCATCGT48750.025.9285138
GCAGAGT324550.025.0672179
TACCCCG7300.024.4727755
GTACATG338350.023.5686571
GTGGTAT48650.023.3887861
TACATGG343700.022.8188932
TAGGCAT69550.022.7124825
GAGTACT131500.022.13052612-13
ACATGGG342300.022.073713
CGTTAAC13650.021.0122411
Produced by FastQC (version 0.11.5)