FastQCFastQC Report
Thu 26 May 2016
ERR580027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7420580
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123300.1661595185282013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106000.14284597699910248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123650.047.4567831
GTATCAA216550.039.0347861
CAACGCA239700.033.6458975
ATCAACG246750.032.7049563
TATCAAC266550.031.3324642
AACGCAG276300.030.071296
TCAACGC282000.028.7995874
GTACTAG53650.027.1681961
ACGCAGA300900.026.7225887
CGCAGAG306600.026.0418558
GTGGTAT50750.023.6085721
GCAGAGT338000.023.1915639
TAGGCAT67350.023.028165
GTACATG343600.022.1438541
GAGTACT137450.021.76176512-13
TACATGG344950.021.6906552
GCATCGT60550.021.1108368
ACATGGG341800.020.9562383
CGACGAG29900.020.90619724-25
CTAGGCA78550.020.5827734
Produced by FastQC (version 0.11.5)