FastQCFastQC Report
Thu 26 May 2016
ERR580026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8760174
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133730.152656785127784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89630.10231531930758452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA220800.041.423161
GGTATCA120400.039.720721
CAACGCA233200.036.5452165
ATCAACG241100.035.3851363
TATCAAC275300.032.9254382
AACGCAG283400.031.4827736
GTACTAG80150.029.7458971
ACGCAGA285400.029.6332177
TAGTACT94250.029.0268824
CGCAGAG290100.028.95868
CTGTGCG96650.028.4409479
GTACATG395600.028.2074681
TAGGCAT96300.027.9210035
TACATGG398400.027.6256712
TCAACGC315000.027.5324334
ACATGGG393400.026.9195793
GGGCACC89050.026.81581994
GCAGAGT317600.025.935319
ACTGTGC112450.025.9053848
GCATCGT84700.025.8499858
Produced by FastQC (version 0.11.5)