FastQCFastQC Report
Thu 26 May 2016
ERR580026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8760174
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119250.13612743308523323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122400.041.7176171
GTATCAA223650.040.4909931
CAACGCA242700.035.4785275
ATCAACG249600.034.4983443
TATCAAC280700.032.110592
AACGCAG293650.030.5682876
GGGCACC86000.028.58155494
ACGCAGA303950.028.1099117
CTGTGCG102050.027.630659
CGCAGAG309150.027.6063738
TAGGCAT96100.027.584535
TCAACGC319050.027.3862764
TAGTACT102750.027.2174554
GTGGTAT35850.026.3619771
GTACATG395250.025.909381
TACATGG396950.025.549532
CTAGGCA108450.025.4402224
ACATGGG390750.025.0437323
GCAGAGT336400.024.8945669
ACTGTGC117200.024.6208788
Produced by FastQC (version 0.11.5)