FastQCFastQC Report
Thu 26 May 2016
ERR580025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5422933
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64250.043.7589071
GTATCAA112950.041.4999281
CAACGCA131200.034.57645
ATCAACG136600.033.212613
TATCAAC142000.032.6129652
AACGCAG153150.030.6531126
TCAACGC155650.029.3281364
ACGCAGA170100.026.2996927
GTACTAG25000.025.9523961
CGCAGAG177200.025.0335438
GTACATG222600.024.3735641
TACATGG228350.022.9776482
GTATAAG34300.022.0683651
GTGGTAT30600.021.8175161
GCAGAGT196900.021.745379
ACATGGG231250.021.6924723
GTGATCG22450.020.7220578
TAGTACT35750.020.120084
CTAGTAC37200.019.7155113
TATTCGC3850.019.53239
Produced by FastQC (version 0.11.5)