FastQCFastQC Report
Thu 26 May 2016
ERR580025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5422933
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64750.044.077781
GTATCAA117900.042.9728931
CAACGCA134100.036.027615
ATCAACG139500.034.532883
TATCAAC148700.033.4694182
AACGCAG157950.031.5676966
TCAACGC160450.030.4920184
ACGCAGA172300.027.793047
CGCAGAG180350.026.292158
GTACATG222500.023.8308981
GCAGAGT198250.023.2307919
TACATGG228850.022.7536982
ACATGGG229050.021.7910123
GTACTAG27100.021.7592951
GTATAAG31250.020.8321061
CGTTAAC10200.020.3496361
CATGGGG182400.019.7111424
GTGATCG18700.019.3508598
AGAGTAC178400.018.93952810-11
GTGGTAT33000.018.2978521
Produced by FastQC (version 0.11.5)