FastQCFastQC Report
Thu 26 May 2016
ERR580024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7257158
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173410.23895028880451546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140800.19401534319633112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76670.10564741734987718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108250.050.742021
GTATCAA197600.040.197171
CAACGCA225250.034.1396035
ATCAACG229550.033.497553
TATCAAC242500.032.4523852
TCAACGC246600.031.3734134
AACGCAG248000.031.3547676
ACGCAGA272150.028.175537
CGCAGAG281750.027.1484328
GTACATG274250.026.8361681
TACATGG284200.025.7718932
ACATGGG280450.025.2392563
GTACTAG33450.025.165741
GTGGTAT43650.024.8874571
GCAGAGT313350.024.0690829
CATGGGG219200.023.2870394
ATGGGGG154650.021.8837935
GAGTACT157250.021.53252412-13
CAGAGTA304350.020.55982810-11
GCATCGT36900.020.1180088
Produced by FastQC (version 0.11.5)