FastQCFastQC Report
Thu 26 May 2016
ERR580024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7257158
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153240.2111570397116888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132150.1820960767286588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74720.10296041508259844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105350.050.5213171
GTATCAA189500.040.914221
CAACGCA226850.032.839815
ATCAACG230700.032.3125883
TATCAAC242200.031.6049462
AACGCAG246150.030.682896
TCAACGC248050.030.1280524
GTACTAG35600.027.3390331
GTGGTAT43000.027.007931
ACGCAGA277850.026.810147
GTACATG279550.026.0361
CGCAGAG286950.025.7795738
TACATGG287100.025.0244732
ACATGGG283150.024.5509173
CATGGGG221400.022.8851784
GCAGAGT319700.022.7844929
ATGGGGG157850.022.0044235
TAGTACT48550.021.7824344
TAGGCAT47500.021.4721855
TGGGGGG139950.020.9216236
Produced by FastQC (version 0.11.5)