FastQCFastQC Report
Thu 26 May 2016
ERR580023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5879829
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148710.2529155184615063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108610.18471625620404947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72060.12255458449556952No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78050.048.1463781
GTATCAA145750.042.691461
CAACGCA170550.035.019685
ATCAACG175850.033.9668123
TATCAAC186800.032.83262
AACGCAG191400.031.8263366
TCAACGC192700.031.2410354
GTACTAG34750.030.0460851
GTACATG218100.029.8449041
TACATGG220000.029.1819652
ACGCAGA205650.029.095087
ACATGGG219150.028.2428073
CGCAGAG213550.027.930478
CATGGGG178650.026.3792154
GCAGAGT239400.024.5801939
GCATCGT37800.024.4957458
ATGGGGG132450.024.4284925
GTGGTAT30600.023.9768921
TAGTACT47100.022.8671344
CTAGGCA51700.022.8339124
Produced by FastQC (version 0.11.5)