FastQCFastQC Report
Thu 26 May 2016
ERR580023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5879829
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132000.22449632463801242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97700.16616129482677133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71470.12155115395362688No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83450.045.739451
GTATCAA142700.039.8257141
CAACGCA170200.032.087635
GTACTAG33600.031.8975121
ATCAACG173500.031.5049423
TATCAAC189150.029.8184072
TCAACGC190200.029.183284
GTACATG213450.029.1797081
AACGCAG192950.029.0830236
TACATGG216550.028.0640182
ACATGGG216900.027.1512583
ACGCAGA209550.026.150937
CATGGGG173750.025.5624074
CGCAGAG216850.025.1838998
TAGGCAT45750.024.9635455
TACCTGG37550.024.6584512
ATGGGGG129200.024.5909465
TAGTACT50250.023.7570444
CTAGGCA49050.023.6675174
GTGGTAT32950.023.5390971
Produced by FastQC (version 0.11.5)