FastQCFastQC Report
Thu 26 May 2016
ERR580022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9775775
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208740.2135278277169841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164390.1681605806189279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100240.1025391848728106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140300.042.907791
GTATCAA239700.039.261141
GTACTAG74500.034.1526831
CAACGCA261350.034.0432745
ATCAACG271350.032.8620453
GCATCGT75300.030.6473168
TATCAAC305350.030.4509982
AACGCAG301800.030.369296
GTACATG366200.029.0379581
TCAACGC312900.028.7977314
TCTAACG33300.028.6711142
TAGGCAT93100.028.2859695
CTAACGC33400.028.0215843
TACATGG372400.027.9488852
CTGTGCG102700.027.2763469
ACGCAGA327700.027.1652647
ACATGGG371150.027.0288433
TAACGCC34250.026.6384814
TAGTACT104800.026.5212824
GGGCACC95400.026.35617894
Produced by FastQC (version 0.11.5)