FastQCFastQC Report
Thu 26 May 2016
ERR580022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9775775
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179040.18314660474489233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147680.1510673066841248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142000.043.1041871
GTATCAA237950.037.537451
CAACGCA269600.031.5030695
GTACTAG69350.031.4534551
ATCAACG278400.030.5071283
GTACATG359700.029.3016431
TATCAAC308900.028.7278522
AACGCAG306250.028.590556
TACATGG365850.028.2000962
CTGTGCG102350.028.0568649
GGGCACC92150.027.95430694
ACATGGG358950.027.5108813
TAGTACT105250.027.3301334
TCAACGC316550.026.9641044
CTAGTAC113300.026.0520653
CATGGGG294300.025.457194
ACGCAGA333650.025.411557
GTACTGT121850.024.8782566
CGCAGAG344650.024.5732338
ATGGGGG226400.023.4801485
Produced by FastQC (version 0.11.5)