FastQCFastQC Report
Thu 26 May 2016
ERR580021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7416602
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197410.2661731073070929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158780.21408725990689537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94760.127767406151766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105600.052.4958571
GTATCAA191800.041.9201281
CAACGCA233200.033.2638055
ATCAACG233600.033.1701773
TATCAAC247100.032.1967122
AACGCAG250900.031.4093176
TCAACGC251300.031.0388494
ACGCAGA278850.027.7549387
GTACTAG37250.027.5172711
CGCAGAG288150.026.842668
GTACATG247500.025.2858751
GTGGTAT44500.024.4077151
TACATGG253650.024.3210812
GCAGAGT318850.023.5983059
ACATGGG253850.023.5413723
GCATCGT35200.022.69458
CTGTGCG47000.022.0988839
TAGTACT48450.022.0284424
GAGTACT182400.021.56044212-13
CTAGTAC49450.021.2983073
Produced by FastQC (version 0.11.5)