FastQCFastQC Report
Thu 26 May 2016
ERR580021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7416602
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175910.23718409050398015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152360.20543100465684958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86600.11676506303021249No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111300.050.6030771
GTATCAA196650.040.8016851
CAACGCA238100.032.825675
ATCAACG239350.032.7336653
TATCAAC251300.031.6063982
TCAACGC257250.030.5099744
AACGCAG259000.030.5013776
GTGGTAT45550.028.6852861
ACGCAGA286900.027.1093397
GTACTAG34950.026.9929661
CGCAGAG296550.026.1162458
TAGTACT52000.024.5836494
CTGTGCG48300.024.2272249
GTACATG251700.023.9880261
GCAGAGT329650.023.1802949
TGGTATC55250.023.137552
TACATGG257250.023.0377712
CTAGTAC52800.022.4315413
ACATGGG253600.022.350673
TAGGCAT48700.021.5207145
Produced by FastQC (version 0.11.5)