##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580021_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7416602
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50216743462842	34.0	31.0	34.0	31.0	34.0
2	32.69913647786412	34.0	31.0	34.0	31.0	34.0
3	32.78055112031089	34.0	31.0	34.0	31.0	34.0
4	36.26318710374374	37.0	37.0	37.0	35.0	37.0
5	36.19229223841322	37.0	37.0	37.0	35.0	37.0
6	36.22224234224784	37.0	37.0	37.0	35.0	37.0
7	36.182646311612785	37.0	37.0	37.0	35.0	37.0
8	36.17225651315791	37.0	37.0	37.0	35.0	37.0
9	37.96062536455374	39.0	38.0	39.0	35.0	39.0
10-11	37.90728921411719	39.0	38.0	39.0	35.0	39.0
12-13	37.87309639913265	39.0	38.0	39.0	35.0	39.0
14-15	39.26721900406682	41.0	39.0	41.0	36.0	41.0
16-17	39.252837889912385	41.0	39.0	41.0	36.0	41.0
18-19	39.25353140157716	41.0	39.0	41.0	36.0	41.0
20-21	39.23743501404012	41.0	39.0	41.0	36.0	41.0
22-23	39.16588924415791	40.5	39.0	41.0	36.0	41.0
24-25	39.09144666250124	40.5	39.0	41.0	35.0	41.0
26-27	38.95353289821942	40.0	38.0	41.0	35.0	41.0
28-29	38.84037663069961	40.0	38.0	41.0	35.0	41.0
30-31	38.717443379056874	40.0	38.0	41.0	35.0	41.0
32-33	38.5644098200227	40.0	38.0	41.0	34.5	41.0
34-35	38.530305724912836	40.0	38.0	41.0	34.5	41.0
36-37	38.57123248625179	40.0	38.0	41.0	35.0	41.0
38-39	38.47221166782308	40.0	38.0	41.0	34.0	41.0
40-41	38.33933909356333	40.0	38.0	41.0	34.0	41.0
42-43	38.30388384060517	40.0	38.0	41.0	34.0	41.0
44-45	38.2040380486913	40.0	38.0	41.0	33.5	41.0
46-47	37.99773224989018	40.0	37.5	41.0	33.0	41.0
48-49	37.776521175061035	40.0	37.0	41.0	33.0	41.0
50-51	37.604542012635974	40.0	37.0	41.0	33.0	41.0
52-53	37.43002981688919	40.0	36.5	41.0	32.0	41.0
54-55	37.230882013083615	39.5	36.0	41.0	32.0	41.0
56-57	37.022488802823716	39.0	35.5	41.0	31.5	41.0
58-59	36.79670966569327	39.0	35.0	41.0	31.0	41.0
60-61	36.540832985240414	38.5	35.0	40.5	31.0	41.0
62-63	36.25859376032312	38.0	35.0	40.0	31.0	41.0
64-65	35.96867332236515	37.5	35.0	40.0	30.5	41.0
66-67	35.64242614070433	37.0	35.0	40.0	30.0	41.0
68-69	35.293294759513856	36.0	34.5	39.0	29.5	41.0
70-71	34.96613739014174	36.0	34.0	39.0	29.0	41.0
72-73	34.61277105067792	35.0	34.0	38.5	29.0	40.0
74-75	34.25759829096937	35.0	34.0	37.5	29.0	39.5
76-77	33.02742671104638	34.5	32.5	36.0	27.0	39.0
78-79	33.65835924591882	35.0	33.5	36.0	29.0	39.0
80-81	33.590696386296585	35.0	34.0	36.0	29.0	38.5
82-83	33.37459027732646	35.0	34.0	36.0	29.0	37.0
84-85	33.14592754201992	35.0	34.0	35.0	29.0	37.0
86-87	32.96033540696939	35.0	33.0	35.0	29.0	36.5
88-89	32.789177173050405	35.0	33.0	35.0	28.0	36.0
90-91	32.62377271963629	35.0	33.0	35.0	27.0	36.0
92-93	32.501818218100425	35.0	33.0	35.0	27.0	35.5
94-95	32.39310488819543	35.0	33.0	35.0	27.0	35.0
96-97	32.289909921012345	35.0	33.0	35.0	26.5	35.0
98-99	32.210372957858596	35.0	33.0	35.0	26.0	35.0
100	30.937022911570555	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.15727415553879354
1101	2	0.09301169303395085
1101	3	0.05730690861489052
1101	4	0.048071701074952955
1101	5	0.03715688057965849
1101	6	0.03680866791432891
1101	7	0.025814150534500868
1101	8	0.04264145600927094
1101	9	0.00971409856504124
1101	10-11	-0.015480642544972056
1101	12-13	-0.013509610973031272
1101	14-15	0.0037363186491035094
1101	16-17	0.029528478856278184
1101	18-19	0.02863791239645508
1101	20-21	-0.0027152335930509253
1101	22-23	-0.026340860960971213
1101	24-25	-0.07171093182766697
1101	26-27	-0.04707149431530411
1101	28-29	-0.03933780732531744
1101	30-31	-0.053786845553830176
1101	32-33	-0.04930185289480704
1101	34-35	-0.10985006060032987
1101	36-37	-0.01374006019942442
1101	38-39	0.017785375095321854
1101	40-41	0.039033975446223224
1101	42-43	-0.06443135984903137
1101	44-45	-0.12350327387237314
1101	46-47	-0.1042637286286805
1101	48-49	-0.17837671615373552
1101	50-51	-0.13563122123890992
1101	52-53	-0.11658722679048594
1101	54-55	-0.15967731020586484
1101	56-57	-0.22136608586941975
1101	58-59	-0.300188626732826
1101	60-61	-0.33837386529028635
1101	62-63	-0.2899436873125012
1101	64-65	-0.2380546811020139
1101	66-67	-0.29304631287579497
1101	68-69	-0.21763432504221925
1101	70-71	-0.2913320356392006
1101	72-73	-0.28007687574908147
1101	74-75	-0.24703419402776206
1101	76-77	-0.1992164507978771
1101	78-79	-0.13084380614699853
1101	80-81	-0.1672415598658148
1101	82-83	-0.16116976655613513
1101	84-85	-0.16916546295244217
1101	86-87	-0.1168796367204692
1101	88-89	-0.11524269788740327
1101	90-91	-0.14468423210227144
1101	92-93	-0.25760942254222385
1101	94-95	-0.2092910460927584
1101	96-97	-0.25353685673565707
1101	98-99	-0.23909330008077845
1101	100	-0.2110551898776052
1102	1	0.12896298288685415
1102	2	0.06391240637528739
1102	3	-0.01950268949183709
1102	4	-0.003497424096060797
1102	5	-0.007294537380474253
1102	6	0.046990904553837254
1102	7	-0.010109474206551283
1102	8	-0.041146562077521764
1102	9	-0.043991979504802714
1102	10-11	0.01818153673329448
1102	12-13	-0.026944640929784214
1102	14-15	0.03756458734493151
1102	16-17	-0.06778810513787903
1102	18-19	-0.014939585995058735
1102	20-21	-0.03350360142296438
1102	22-23	-0.03534660605966167
1102	24-25	-0.05830380839891802
1102	26-27	-0.10370398904111511
1102	28-29	-0.09213112106144905
1102	30-31	-0.09865489816057504
1102	32-33	-0.1715102184585433
1102	34-35	-0.07867669837696667
1102	36-37	-0.03228759413180171
1102	38-39	-0.057815915925168326
1102	40-41	-0.030781601669190195
1102	42-43	0.008523158259919228
1102	44-45	-0.046353451015725966
1102	46-47	-0.03420735253394014
1102	48-49	-0.11368554535849285
1102	50-51	-0.12299743467925595
1102	52-53	-0.16975906663562057
1102	54-55	-0.20286853457042042
1102	56-57	-0.20873659397016553
1102	58-59	-0.18796376897197575
1102	60-61	-0.23341492434722255
1102	62-63	-0.2235905355295955
1102	64-65	-0.27049020019428127
1102	66-67	-0.26882732053424263
1102	68-69	-0.18488017720661531
1102	70-71	-0.27802500409164566
1102	72-73	-0.1910751749934505
1102	74-75	-0.2374404485678312
1102	76-77	-0.24989961382192405
1102	78-79	-0.1949981794505149
1102	80-81	-0.20235743786688687
1102	82-83	-0.1370162809326203
1102	84-85	-0.12544820404100676
1102	86-87	-0.13584817007676264
1102	88-89	-0.1330439250173754
1102	90-91	-0.11595637221603994
1102	92-93	-0.16731816673723898
1102	94-95	-0.2056251590325786
1102	96-97	-0.19664953964961995
1102	98-99	-0.23976540566898308
1102	100	-0.1705181182287845
1103	1	0.14646814865415791
1103	2	0.08402464365423867
1103	3	0.08574787801683925
1103	4	0.015897808988604822
1103	5	0.020913306153772737
1103	6	0.05590249001588177
1103	7	0.011314702862527781
1103	8	0.023778875053942272
1103	9	-0.009257667972221384
1103	10-11	-0.04326520796926303
1103	12-13	-0.015518060657058186
1103	14-15	-4.077023128559176E-4
1103	16-17	0.01890048928843413
1103	18-19	0.010285814582353225
1103	20-21	0.004533653170909702
1103	22-23	-0.1287870016543593
1103	24-25	-0.11119474717963129
1103	26-27	-0.10898666871507601
1103	28-29	-0.07407625266214524
1103	30-31	-0.1588990780779227
1103	32-33	-0.17284520806362735
1103	34-35	-0.20193735653960942
1103	36-37	-0.09840504723457855
1103	38-39	-0.11173623510479302
1103	40-41	-0.16069798362876497
1103	42-43	-0.15346123278708035
1103	44-45	-0.1307774733455247
1103	46-47	-0.15969140302496498
1103	48-49	-0.159608419154452
1103	50-51	-0.08998255355064799
1103	52-53	-0.1136516588050327
1103	54-55	-0.09752571527673837
1103	56-57	-0.115006092861762
1103	58-59	-0.06979866940675095
1103	60-61	-0.10427120580085614
1103	62-63	-0.13765399712401916
1103	64-65	-0.10283629877717715
1103	66-67	-0.16813128440558245
1103	68-69	-0.14037026669662112
1103	70-71	-0.16317346643525354
1103	72-73	-0.1983583124771826
1103	74-75	-0.27738199605799707
1103	76-77	-0.23680851825695015
1103	78-79	-0.1532961355928677
1103	80-81	-0.11031783721679034
1103	82-83	-0.05134011001515404
1103	84-85	-0.10016879003470791
1103	86-87	-0.05887575110190113
1103	88-89	-0.06264197266215632
1103	90-91	-0.10307346033861364
1103	92-93	-0.04764962920413751
1103	94-95	-0.02399871891813632
1103	96-97	-0.028216146541431897
1103	98-99	-0.06609344531277372
1103	100	-0.08323026363062525
1104	1	0.07183618623304966
1104	2	0.04060887835971272
1104	3	0.02609847829147327
1104	4	0.03006778503318941
1104	5	-0.0051090487666343165
1104	6	0.006862984227176128
1104	7	0.0072002313782491
1104	8	-0.0038698923202247215
1104	9	-0.02429374106707627
1104	10-11	-0.00763230857513264
1104	12-13	-0.012432468836408361
1104	14-15	-0.030831322466227107
1104	16-17	-0.024599328870458237
1104	18-19	-0.05120835004404256
1104	20-21	-0.06032122980310106
1104	22-23	-0.08216961807691803
1104	24-25	-0.12465822364449508
1104	26-27	-0.055346970254035455
1104	28-29	-0.13179895514185347
1104	30-31	-0.12235905873947672
1104	32-33	-0.16513646436318652
1104	34-35	-0.17478037521446055
1104	36-37	-0.14474538447295515
1104	38-39	-0.1095058846696233
1104	40-41	-0.09277691638916963
1104	42-43	-0.11000313563572917
1104	44-45	-0.13501992648561156
1104	46-47	-0.16591183857308067
1104	48-49	-0.1954319384374088
1104	50-51	-0.17937008706577018
1104	52-53	-0.24117885145995643
1104	54-55	-0.3284970787660271
1104	56-57	-0.21201138843655087
1104	58-59	-0.2697217931490812
1104	60-61	-0.32037120734717917
1104	62-63	-0.24303360907553895
1104	64-65	-0.2632728493886418
1104	66-67	-0.28233320088768465
1104	68-69	-0.20759992233433877
1104	70-71	-0.21159629367132027
1104	72-73	-0.2367181129800855
1104	74-75	-0.22330191015373657
1104	76-77	-0.2900799705777075
1104	78-79	-0.12627082719862415
1104	80-81	-0.11249952041519151
1104	82-83	-0.11604760377829848
1104	84-85	-0.08680522233687782
1104	86-87	-0.1338059737857904
1104	88-89	-0.12463665848263616
1104	90-91	-0.07296906130559222
1104	92-93	-0.15182747368589133
1104	94-95	-0.13490243311577643
1104	96-97	-0.14071441303836707
1104	98-99	-0.193101930917166
1104	100	-0.12029696596623651
1105	1	0.1438379099934437
1105	2	0.09555942964622233
1105	3	0.08058120926845191
1105	4	0.049662414280298606
1105	5	0.023788372961810467
1105	6	0.023884768056625205
1105	7	0.04562752056373398
1105	8	-0.007421297330225229
1105	9	0.014034092324685332
1105	10-11	0.025034211177484167
1105	12-13	0.04693357311882096
1105	14-15	0.1114087976368836
1105	16-17	0.07891986940591522
1105	18-19	0.044943522908788225
1105	20-21	0.04658953006401134
1105	22-23	0.033325986666646656
1105	24-25	0.034873306660202275
1105	26-27	0.02542952184104763
1105	28-29	0.04529741003069887
1105	30-31	0.040332722294635914
1105	32-33	-0.022878674315734315
1105	34-35	0.018198924432610397
1105	36-37	0.04307420390748007
1105	38-39	0.07355430113872785
1105	40-41	0.07482039651902994
1105	42-43	0.08573455697236199
1105	44-45	0.14484757431210937
1105	46-47	0.11863768111774675
1105	48-49	0.13785688892417625
1105	50-51	0.08176800788143623
1105	52-53	0.06454879229716681
1105	54-55	0.17133976087121283
1105	56-57	0.13763728686023313
1105	58-59	0.11850920124607711
1105	60-61	0.09107174008705243
1105	62-63	0.09608195026002875
1105	64-65	0.11334401178160647
1105	66-67	0.0944269539917002
1105	68-69	0.09842439970078942
1105	70-71	0.09147188780872284
1105	72-73	0.0331688011264859
1105	74-75	0.010864016837452084
1105	76-77	0.041232938668798624
1105	78-79	0.06355397167120458
1105	80-81	0.1179111189392259
1105	82-83	0.13070712146588903
1105	84-85	0.19107071207410797
1105	86-87	0.1065935489944323
1105	88-89	0.13407381015859698
1105	90-91	0.1963599236519329
1105	92-93	0.16490326804393618
1105	94-95	0.07452424151622239
1105	96-97	0.06080514966421191
1105	98-99	0.05488921783226175
1105	100	0.09170408565363175
1106	1	0.06676973883882198
1106	2	0.04619265257226601
1106	3	-0.031791811474874976
1106	4	0.019732182664881748
1106	5	-0.008606108780710997
1106	6	0.03605805406812834
1106	7	0.005158285342091062
1106	8	0.015378825489165138
1106	9	0.0170892903963491
1106	10-11	-0.021232242992141437
1106	12-13	-0.027702225967637162
1106	14-15	-0.038213774375265075
1106	16-17	-0.07444978851300021
1106	18-19	-0.028042356800874302
1106	20-21	-0.07017452913619593
1106	22-23	-0.09761557877631333
1106	24-25	-0.0706302506632639
1106	26-27	-0.04043949584632145
1106	28-29	-0.05670608351038453
1106	30-31	-0.08468572294037102
1106	32-33	-0.10491926232540294
1106	34-35	-0.11861505050217858
1106	36-37	-0.08259750076493333
1106	38-39	-0.07688518443817571
1106	40-41	-0.16451556101215203
1106	42-43	-0.11403059953000394
1106	44-45	-0.07229059899368906
1106	46-47	-0.0889871723513096
1106	48-49	-0.06588926892045777
1106	50-51	-0.07786960661713493
1106	52-53	-0.0494808368746007
1106	54-55	-0.03458439246251288
1106	56-57	-0.07404364467600999
1106	58-59	-0.10190376189085981
1106	60-61	-0.1188072362323922
1106	62-63	-0.16676588370860657
1106	64-65	-0.12746461401314946
1106	66-67	-0.12013735279096949
1106	68-69	-0.1315025311653244
1106	70-71	-0.11816858529634544
1106	72-73	-0.12426017432181169
1106	74-75	-0.09344968602629677
1106	76-77	-0.11973039508450256
1106	78-79	-0.06494602863522658
1106	80-81	-0.04279534297358367
1106	82-83	-0.013687203731819864
1106	84-85	0.0461688682160144
1106	86-87	0.03433144393412135
1106	88-89	0.0325869495485307
1106	90-91	0.04591177032486371
1106	92-93	-0.031238917729758953
1106	94-95	-0.0380990845901934
1106	96-97	-0.009027665194658141
1106	98-99	0.04132475791933388
1106	100	0.14143462806480045
1107	1	0.0525310516058326
1107	2	0.03732321135424144
1107	3	-0.006233257217495236
1107	4	-0.03499746479539567
1107	5	-0.01569755149226637
1107	6	-0.006061210544565654
1107	7	-0.03773216992184558
1107	8	-0.10700681582733296
1107	9	-0.12386775091940194
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2316	22-23	-0.04830274461791362
2316	24-25	-0.031301816760432644
2316	26-27	-0.019292792470018583
2316	28-29	0.02380327335377075
2316	30-31	-0.4008590895328936
2316	32-33	-0.17161846965638716
2316	34-35	-0.26294658907365687
2316	36-37	-0.5684855377277813
2316	38-39	-0.3523542410773146
2316	40-41	-0.3003454962606611
2316	42-43	-0.3721221978366245
2316	44-45	-0.24482580709636892
2316	46-47	-0.3642401564734996
2316	48-49	-0.29714285967668275
2316	50-51	-0.2832842810975862
2316	52-53	-0.2869330523921221
2316	54-55	-0.16471843946242615
2316	56-57	-0.9166532381715626
2316	58-59	-0.7705228466268679
2316	60-61	-0.3715162240662906
2316	62-63	-0.16253631914094058
2316	64-65	-0.12189679385660668
2316	66-67	-0.058922684177474594
2316	68-69	-0.16017788643722497
2316	70-71	-0.16100067391590756
2316	72-73	-0.0023172310959367337
2316	74-75	-0.06350075065513039
2316	76-77	-0.14331905374099563
2316	78-79	-0.10133210772432477
2316	80-81	-0.14210366251315776
2316	82-83	-0.2961434113163648
2316	84-85	-0.13138266564718748
2316	86-87	-0.20396223116252798
2316	88-89	-0.21895759347847843
2316	90-91	-0.08839298368724258
2316	92-93	-0.15686765296399585
2316	94-95	-0.09560083262608998
2316	96-97	-0.23418477188699427
2316	98-99	-0.10169590694143693
2316	100	-0.37879894564781225
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	4.0
12	13.0
13	75.0
14	315.0
15	923.0
16	2415.0
17	5190.0
18	9561.0
19	15091.0
20	22609.0
21	32943.0
22	43227.0
23	45212.0
24	43706.0
25	44125.0
26	49285.0
27	59220.0
28	73273.0
29	90081.0
30	110734.0
31	137237.0
32	173589.0
33	227788.0
34	330349.0
35	501428.0
36	895557.0
37	1820256.0
38	2192127.0
39	490135.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04175410317878	19.529942934651707	13.60675409505984	23.821548867109673
2	15.91187613774093	19.97750709526963	40.0242376486892	24.086379118300247
3	19.001656987981747	24.927886842107746	29.818313809442206	26.252142360468305
4	12.84981576916693	16.498040655504994	38.20226249243074	32.449881082897335
5	13.491724254171583	37.61375358166189	34.834437889769085	14.060084274397438
6	31.644329842696155	36.92789501175876	17.417275458491638	14.010499687053452
7	27.686875800316507	32.19596534637112	21.92401717391413	18.19314167939825
8	26.097553806442424	35.28011008841806	19.764233776628163	18.858102328511347
9	26.119697814673902	15.63496780637875	20.51914573910492	37.72618863984242
10-11	25.224668402503198	25.924868238088166	27.885673173289582	20.96479018611905
12-13	25.688922902698412	23.49265913180785	28.100325816609523	22.718092148884214
14-15	23.01986691032799	25.078949814931505	25.739658792797783	26.161524481942717
16-17	22.432860326260407	27.87360122730241	26.578848692074295	23.11468975436289
18-19	22.04779293190244	27.27559739479455	28.624448125108543	22.05216154819447
20-21	23.14780213946104	26.41096730807801	28.460097045979705	21.98113350648125
22-23	23.05812374689716	26.248963472431402	28.141018985814238	22.551893794857207
24-25	22.686467468525343	26.316303611815762	28.493527089629456	22.503701830029442
26-27	22.666020355432977	26.567119904779208	28.102545052735827	22.664314687051988
28-29	22.325330547199304	26.767147558416543	28.352431031619567	22.555090862764583
30-31	22.92716290841378	26.45209331550784	28.266383238643833	22.354360537434545
32-33	22.827300541500605	26.65918378919349	27.906082808038434	22.607432861267466
34-35	22.93356652904389	26.679014172774963	28.185553754971227	22.201865543209923
36-37	22.85626517942032	26.451070155664212	28.036698217633234	22.655966447282232
38-39	22.80173985193688	26.579797681631312	28.20690216424274	22.411560302189066
40-41	22.859458825660056	26.194990004511435	28.342302381514738	22.603248788313763
42-43	22.65550310848453	26.30441600862118	28.105464099046486	22.9346167838478
44-45	22.721678019385276	26.681838232304962	27.996763435286336	22.59972031302343
46-47	22.731374932782177	26.561765963861756	27.580968821178754	23.125890282177313
48-49	22.959795654916043	26.5702548825187	27.664738188255143	22.805211274310107
50-51	22.872698856356294	26.6786060697464	27.46918476426275	22.979510309634556
52-53	22.727444035721078	26.87476702127638	27.35126560750974	23.0465233354928
54-55	22.593839208988186	26.774201638758704	27.461040478421427	23.170918673831682
56-57	22.980865526092696	26.72297738009563	27.159674176297386	23.13648291751429
58-59	22.559994984195836	26.859759396458532	27.544275089891773	23.035970529453863
60-61	22.7398685996534	26.978033379576626	27.266070802800918	23.01602721796906
62-63	22.716357619586343	26.74250352371448	27.210188262456004	23.330950594243173
64-65	22.418131706850748	26.729911925676763	27.297680587688905	23.554275779783584
66-67	22.60712406697828	26.651568787084575	27.2569761399389	23.484331005998254
68-69	22.488012987859644	26.65714910386209	27.288730364717583	23.566107543560683
70-71	22.425717997112937	26.39888120245926	27.331614740794013	23.843786059633786
72-73	22.583813597921797	26.490703438515574	27.304144141131587	23.621338822431053
74-75	22.623558763143496	26.684527535367327	27.14032421137682	23.55158949011236
76-77	22.46976770840005	26.578939029775526	27.019561575308526	23.931731686515896
78-79	22.39981328061828	26.589990099072647	27.06274151460323	23.94745510570585
80-81	22.262538108928805	26.814777830557503	27.078363324065347	23.84432073644835
82-83	22.58764277462508	26.428575473560333	27.469014036650837	23.514767715163746
84-85	22.715612268005632	26.520106543826692	26.951820867545155	23.812460320622517
86-87	22.12703526754316	26.81349026135133	27.201794336309153	23.85768013479636
88-89	22.387402732474115	26.287670319507317	27.199620897582548	24.12530605043602
90-91	22.35179208345182	26.279991710477223	27.196268474289603	24.171947731781355
92-93	22.374799651531536	26.315659251127187	27.185846118083674	24.123694979257603
94-95	21.9475619726646	26.273353033488355	27.39870377016093	24.38038122368611
96-97	22.031519259990915	26.168512304978282	27.327355225704697	24.47261320932611
98-99	21.79934600640591	26.304159539757215	27.226294147989055	24.670200305847818
100	22.012314370253073	26.06941719244008	27.02946830095993	24.888800136346916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	794.0
1	549.5
2	428.5
3	587.0
4	484.5
5	442.0
6	786.0
7	1529.0
8	2577.0
9	3288.5
10	3087.0
11	2761.0
12	3022.5
13	3482.0
14	4051.5
15	4745.0
16	5506.0
17	6242.5
18	6975.5
19	7745.0
20	8537.0
21	9499.0
22	10753.5
23	12789.5
24	15476.0
25	18746.0
26	23557.5
27	30014.0
28	37797.0
29	48823.0
30	63065.0
31	77725.5
32	95664.0
33	114644.5
34	132011.5
35	149649.5
36	169356.5
37	193828.5
38	209326.5
39	214665.5
40	217323.0
41	218125.5
42	225380.0
43	238829.5
44	253262.5
45	274734.5
46	295883.5
47	310134.0
48	321825.5
49	325461.0
50	319998.5
51	316844.5
52	311568.5
53	299322.5
54	286138.5
55	261248.0
56	232252.0
57	199944.0
58	169065.5
59	149704.0
60	125151.5
61	97635.5
62	75808.0
63	58177.5
64	41674.5
65	27562.0
66	19140.0
67	13620.5
68	10045.5
69	7523.0
70	5564.5
71	4064.5
72	2918.0
73	2068.0
74	1498.5
75	1004.5
76	631.5
77	356.0
78	194.0
79	110.0
80	62.5
81	36.0
82	29.0
83	24.0
84	13.0
85	8.5
86	8.5
87	5.5
88	3.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35700985437805616
2	5.528138088035464E-4
3	0.001793274062704187
4	0.0012269769902712858
5	0.004746108797532887
6	0.0
7	6.741631814677395E-5
8	2.4269874532838623E-4
9	0.003047217580234183
10-11	6.741631814677395E-5
12-13	8.764121359080614E-5
14-15	3.168566952898376E-4
16-17	1.483158999229027E-4
18-19	8.898953995374161E-4
20-21	1.9550732262564444E-4
22-23	1.617991635522575E-4
24-25	0.0
26-27	0.002083164230735315
28-29	0.011197850444179154
30-31	0.003465198752744181
32-33	0.013557421579316243
34-35	0.010779869271669155
36-37	0.012519210279855923
38-39	0.012316961325415602
40-41	0.013570904842945597
42-43	0.0060405021059509465
44-45	0.0013011349402327374
46-47	0.005582071142552884
48-49	0.012283253166342215
50-51	0.003539356702705633
52-53	0.0056697123561436896
54-55	0.005110156915525466
56-57	1.9550732262564444E-4
58-59	6.000052315062881E-4
60-61	4.1798117250999847E-4
62-63	9.101202949814484E-4
64-65	3.370815907338698E-4
66-67	0.0014561924719703175
68-69	0.001853948749036284
70-71	9.168619267961258E-4
72-73	0.0027505857803883775
74-75	0.0077663598505083595
76-77	0.003734864025331277
78-79	0.001685407953669349
80-81	4.4494769976870805E-4
82-83	2.4269874532838623E-4
84-85	0.0016651830582253167
86-87	0.005096673651896111
88-89	0.0018404654854069287
90-91	1.483158999229027E-4
92-93	0.0020090062807738636
94-95	0.003337107748265311
96-97	0.0020561977034766056
98-99	0.002271929921546282
100	0.003047217580234183
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7416602.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.386219760687986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.85209267458836	45.20034917795077
2	14.562212877897649	17.58713974093296
3	4.935905666136009	8.941820528199422
4	2.117867964497156	5.115601613129848
5	1.0913664409617763	3.2951746871678864
6	0.6404145757511137	2.3203329185552715
7	0.4040627272336883	1.7079874450685497
8	0.26149166567534166	1.2632394551247628
9	0.1867505526118934	1.0149443919406549
>10	0.8926467304437637	9.24029339805765
>50	0.038717964767102445	1.5750490855921666
>100	0.015548031350256248	1.5843308431396155
>500	6.237981110148228E-4	0.26230442351016187
>1k	2.1696725463169104E-4	0.21198764673186288
>5k	2.7120906754011358E-5	0.14182733446190382
>10k+	5.4241813508022717E-5	0.5376173104365954
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17591	0.23718409050398015	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15236	0.20543100465684958	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8660	0.11676506303021249	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.3933054517419164E-5
7	0.0	0.0	0.0	0.0	5.3933054517419164E-5
8	0.0	0.0	0.0	0.0	5.3933054517419164E-5
9	0.0	0.0	0.0	0.0	8.089958177612875E-5
10-11	0.0	0.0	0.0	2.6966527258709582E-5	9.438284540548354E-5
12-13	0.0	0.0	0.0	2.6966527258709582E-5	1.0786610903483833E-4
14-15	0.0	0.0	0.0	4.0449790888064375E-5	1.2134937266419312E-4
16-17	0.0	0.0	0.0	4.719142270274177E-5	1.3483263629354792E-4
18-19	0.0	0.0	0.0	5.3933054517419164E-5	1.752824271816123E-4
20-21	2.0224895444032187E-5	0.0	0.0	6.741631814677396E-5	1.752824271816123E-4
22-23	4.0449790888064375E-5	0.0	0.0	2.4944037714306366E-4	2.0224895444032187E-4
24-25	4.0449790888064375E-5	0.0	0.0	6.4719665420903E-4	2.4269874532838623E-4
26-27	4.0449790888064375E-5	0.0	0.0	0.001483158999229027	2.4269874532838623E-4
28-29	4.0449790888064375E-5	0.0	0.0	0.0048741998020117566	2.4269874532838623E-4
30-31	4.0449790888064375E-5	0.0	0.0	0.01806757326333542	2.5618200895774105E-4
32-33	4.0449790888064375E-5	0.0	0.0	0.048168959315869994	2.6966527258709584E-4
34-35	4.0449790888064375E-5	0.0	0.0	0.09499633390061918	2.6966527258709584E-4
36-37	4.0449790888064375E-5	0.0	0.0	0.16911383407118247	2.7640690440177324E-4
38-39	4.0449790888064375E-5	0.0	0.0	0.2723484420493374	2.8314853621645063E-4
40-41	4.0449790888064375E-5	0.0	0.0	0.3813269203335975	3.101150634751602E-4
42-43	4.0449790888064375E-5	0.0	0.0	0.4873053724603262	3.101150634751602E-4
44-45	4.0449790888064375E-5	0.0	0.0	0.6203110265320965	3.101150634751602E-4
46-47	4.0449790888064375E-5	0.0	0.0	0.7754157496923794	3.101150634751602E-4
48-49	4.0449790888064375E-5	0.0	0.0	0.9427160848054136	3.370815907338698E-4
50-51	4.0449790888064375E-5	0.0	0.0	1.11174901929482	3.370815907338698E-4
52-53	4.0449790888064375E-5	0.0	0.0	1.27600213682762	3.7753138162193415E-4
54-55	4.0449790888064375E-5	0.0	0.0	1.4599543025229074	4.179811725099985E-4
56-57	4.0449790888064375E-5	0.0	0.0	1.6763067507195344	4.247228043246759E-4
58-59	4.0449790888064375E-5	0.0	0.0	1.9160324364176478	4.314644361393533E-4
60-61	4.0449790888064375E-5	0.0	0.0	2.158879497645957	4.449476997687081E-4
62-63	4.0449790888064375E-5	0.0	0.0	2.406054686499289	4.719142270274177E-4
64-65	4.0449790888064375E-5	0.0	0.0	2.660558029135175	4.786558588420951E-4
66-67	4.0449790888064375E-5	0.0	0.0	2.9470369314680767	4.8539749065677247E-4
68-69	4.0449790888064375E-5	0.0	0.0	3.261095849554823	4.8539749065677247E-4
70-71	4.0449790888064375E-5	0.0	0.0	3.59782687543433	4.8539749065677247E-4
72-73	4.0449790888064375E-5	0.0	0.0	3.9369916303989347	5.79780336062256E-4
74-75	4.0449790888064375E-5	0.0	0.0	4.274302436614503	5.79780336062256E-4
76-77	4.0449790888064375E-5	0.0	0.0	4.641728112146236	5.79780336062256E-4
78-79	4.0449790888064375E-5	0.0	0.0	5.044763086923095	5.865219678769334E-4
80-81	4.719142270274177E-5	0.0	0.0	5.474973579544918	6.269717587649978E-4
82-83	5.3933054517419164E-5	0.0	0.0	5.915397644365978	6.80904813282417E-4
84-85	5.3933054517419164E-5	0.0	0.0	6.34802838280927	6.876464450970944E-4
86-87	5.3933054517419164E-5	0.0	0.0	6.802522233227561	6.943880769117718E-4
88	5.3933054517419164E-5	0.0	0.0	7.162323123176894	7.011297087264492E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11130	0.0	50.603077	1
GTATCAA	19665	0.0	40.801685	1
CAACGCA	23810	0.0	32.82567	5
ATCAACG	23935	0.0	32.733665	3
TATCAAC	25130	0.0	31.606398	2
TCAACGC	25725	0.0	30.509974	4
AACGCAG	25900	0.0	30.501377	6
GTGGTAT	4555	0.0	28.685286	1
ACGCAGA	28690	0.0	27.109339	7
GTACTAG	3495	0.0	26.992966	1
CGCAGAG	29655	0.0	26.116245	8
TAGTACT	5200	0.0	24.583649	4
CTGTGCG	4830	0.0	24.227224	9
GTACATG	25170	0.0	23.988026	1
GCAGAGT	32965	0.0	23.180294	9
TGGTATC	5525	0.0	23.13755	2
TACATGG	25725	0.0	23.037771	2
CTAGTAC	5280	0.0	22.431541	3
ACATGGG	25360	0.0	22.35067	3
TAGGCAT	4870	0.0	21.520714	5
>>END_MODULE