FastQCFastQC Report
Thu 26 May 2016
ERR580020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7841153
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102870.13119244070355468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79630.10155394238576902No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97250.039.1712231
GTATCAA156550.036.049551
GTACTAG65350.035.2632371
TCTAACG32600.032.7480552
CAACGCA160550.032.7462545
GCATCGT65800.031.9997548
CTAACGC32950.031.971393
TAACGCC33850.031.3990084
GTACATG281200.031.0743541
ATCAACG170200.030.8647353
TAGTACT88950.030.8239844
CTGTGCG90950.030.4383379
GGGCACC81600.030.0657594
TACATGG291350.029.3626272
CGCCTAC36100.029.0332017
ACATGGG284500.028.8953573
TATCAAC195700.028.5497842
AACGCAG196650.028.0346936
TAGGCAT85350.027.934095
CTAGTAC100250.027.9126783
Produced by FastQC (version 0.11.5)