FastQCFastQC Report
Thu 26 May 2016
ERR580020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7841153
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87880.11207535422405351No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA155950.038.1042141
GGTATCA94600.037.17251
CAACGCA164350.033.116115
GTACTAG59300.031.7114871
ATCAACG173250.031.4703733
CTGTGCG88500.030.6952539
GGGCACC79200.030.21194
TAGTACT90350.029.5482484
TATCAAC201450.029.188382
AACGCAG199800.028.6742536
GTACATG279250.028.232621
TACATGG283000.027.5703052
ACATGGG275500.027.1605513
CTAGTAC101350.026.8517073
CTAACGC32100.026.5027353
TCTAACG33300.026.3947322
TAACGCC32500.026.176214
TCAACGC212050.026.0663764
GTACTGT108250.026.049976
ACTGTGC117100.025.8070268
Produced by FastQC (version 0.11.5)