FastQCFastQC Report
Thu 26 May 2016
ERR580018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8250241
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167520.20304861397382212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133070.1612922580079782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83460.10116068124555393No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120250.041.496461
GTATCAA202000.037.3218231
CAACGCA206950.034.1309435
GGGCACC86700.033.82501694
GTACTAG80850.033.6805571
ATCAACG221250.032.0559963
TAGTACT109400.031.03834
CTGTGCG108200.030.9727339
CTAACGC40100.030.846273
GTACATG332900.030.699171
GCATCGT83150.030.636648
TAGGCAT97850.030.3738655
TCTAACG41350.030.2550142
TAACGCC40700.030.1595024
TACATGG334850.029.8890842
TATCAAC251350.029.695412
ACATGGG326700.029.3390663
AACGCAG251600.028.9180956
CGCCTAC43000.028.3100517
CTAGGCA109300.027.8825764
Produced by FastQC (version 0.11.5)