FastQCFastQC Report
Thu 26 May 2016
ERR580018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8250241
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150250.1821158921296966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124990.15149860470742613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83120.10074857207201583No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123400.039.8870281
GTATCAA208050.037.803181
GGGCACC88350.035.7547694
CAACGCA218750.033.7114645
ATCAACG230000.032.1041833
TAGTACT113350.030.7259044
TAGGCAT97050.030.413535
CTGTGCG114850.030.4044289
CTAACGC37350.030.2018763
TAACGCC38300.029.6978194
TATCAAC264900.029.489692
TCTAACG39250.029.4589062
AACGCAG264850.028.995716
CTAGGCA105850.028.995484
GTACATG327750.028.80191
TACATGG328200.028.3848252
ACATGGG320950.027.9271473
CGCCTAC40600.027.8976387
GTACTGT128250.027.8141676
ACTGTGC134700.027.6684388
Produced by FastQC (version 0.11.5)