FastQCFastQC Report
Thu 26 May 2016
ERR580017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7076208
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193510.27346567540128836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146200.20660783289581083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92720.1310306310950724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94750.047.4515571
GTATCAA170050.041.8164331
CAACGCA195700.034.8677445
ATCAACG201400.033.8837973
TATCAAC214350.032.6933332
GTACTAG46150.031.998511
AACGCAG221400.031.5478926
GTACATG245850.030.7409631
TCAACGC226950.030.4620364
TACATGG252350.029.3917052
ACATGGG244650.028.9702433
ACGCAGA241350.028.2384367
CGCAGAG248900.027.3246358
GCATCGT48250.027.2754218
GTGGTAT35800.027.1931271
CATGGGG207650.027.019694
TAGTACT65400.026.5350824
CTAGGCA60950.026.3119184
CTGTGCG65200.026.0958659
TAGGCAT58950.025.9266745
Produced by FastQC (version 0.11.5)