FastQCFastQC Report
Thu 26 May 2016
ERR580017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7076208
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170060.24032645733421065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137310.1940446069420232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92990.13141219138838203No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97850.046.2659341
GTATCAA173150.039.8293761
CAACGCA199200.032.9624945
ATCAACG205450.032.0743563
TATCAAC219950.030.9018822
GTACTAG45350.030.683881
AACGCAG227000.029.524476
GTACATG244250.029.5055311
TCAACGC231900.028.6795124
TACATGG246900.028.5759182
ACATGGG244700.027.8900133
TAGTACT62350.026.4598084
ACGCAGA248650.026.4245247
CTAACGC17650.026.3637123
CATGGGG205750.025.5169874
CGCAGAG257750.025.5100068
TCTAACG18950.025.3006442
CTGTGCG64150.025.2763279
CTAGTAC68750.025.227243
TAGGCAT59750.024.7791085
Produced by FastQC (version 0.11.5)