FastQCFastQC Report
Thu 26 May 2016
ERR580016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6515293
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109380.16788193562438405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83120.1275767643911026No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79300.039.9767721
GTATCAA134100.038.161121
CAACGCA142950.033.9862945
ATCAACG149550.032.6788563
GTACATG211350.031.8017461
TATCAAC164950.030.8828352
GTACTAG40450.030.814021
TACATGG214300.030.7727892
AACGCAG164200.030.3147226
ACATGGG216150.029.529923
TCAACGC168750.029.1272964
ACGCAGA178000.027.2512937
CATGGGG178050.027.1303964
TAGTACT56750.026.3565734
CTGTGCG59250.026.0990689
CGCAGAG188300.025.6604088
GCATCGT43150.025.5981048
ATGGGGG127100.025.3103525
CTAGTAC64750.023.3912683
TAGGCAT55550.023.3675215
Produced by FastQC (version 0.11.5)