FastQCFastQC Report
Thu 26 May 2016
ERR580016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6515293
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99900.15333155393011488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78450.12040901307124638No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75500.040.3481861
GTATCAA128800.038.6523671
CAACGCA142250.033.172445
ATCAACG147650.031.9913023
GTACATG205400.030.2341921
TATCAAC165800.029.8506072
GTACTAG40350.029.8258231
AACGCAG163750.029.3894396
TCAACGC164400.028.9889264
TACATGG210500.028.959912
ACATGGG210300.027.869333
TACCTGG43200.026.8733942
TAGTACT55650.026.772574
ACGCAGA178900.026.3752167
CATGGGG170250.025.5634734
TAGGCAT52850.025.523075
CTAACGC16950.025.510413
CTAGTAC59650.025.3714163
CTGTGCG57700.024.9242219
CGCAGAG190150.024.8149498
Produced by FastQC (version 0.11.5)