FastQCFastQC Report
Thu 26 May 2016
ERR580015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8350898
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218160.2612413658986135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174350.20877994198947228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102740.1230286850587805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131400.049.097671
GTATCAA235900.039.666811
CAACGCA261450.034.191515
ATCAACG264000.033.806223
GTACTAG62650.031.7483711
TATCAAC293450.031.4939062
AACGCAG295100.031.0757926
TCAACGC305000.029.6638374
ACGCAGA312450.028.4640927
CGCAGAG317050.027.9911678
GCATCGT59900.027.6078538
TAGGCAT74500.026.433475
GTACATG321350.025.841311
GGGCACC64800.025.6789294
GTGGTAT48750.025.3678281
CTGTGCG75850.025.3371289
TACATGG322450.025.1480222
ACATGGG316850.024.9338653
CTAACGC27650.024.8160883
GCAGAGT349950.024.8133649
Produced by FastQC (version 0.11.5)