FastQCFastQC Report
Thu 26 May 2016
ERR580015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8350898
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190390.22798745715730215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162110.1941228356519263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98080.11744844686164291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128150.048.6084251
GTATCAA230150.039.8938141
CAACGCA263150.033.3626335
ATCAACG266250.032.9920353
TATCAAC294000.031.023222
AACGCAG298250.030.270766
TCAACGC307450.028.953094
CTGTGCG71750.027.771689
ACGCAGA318600.027.466787
CGCAGAG321600.027.1376538
GGGCACC68800.026.23189294
TAGGCAT72100.026.0742345
GTACATG310900.025.812321
TAGTACT80600.025.7736934
TACATGG314800.025.090372
GTGGTAT45800.024.9433671
ACATGGG305900.024.8131833
CTAACGC28500.024.4065463
GCAGAGT358300.024.0684159
TAACGCC29250.023.6200544
Produced by FastQC (version 0.11.5)