FastQCFastQC Report
Thu 26 May 2016
ERR580014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8408757
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107720.12810454624863105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87030.1034992448943405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110250.036.6817861
GTATCAA176750.035.5715331
GTACTAG76000.034.8357661
CAACGCA177250.032.787915
ATCAACG186900.031.1492163
GGGCACC90150.030.54967594
TAGTACT106900.029.2085914
GTACATG310450.029.0527671
GCATCGT81250.028.6323228
CTGTGCG110900.028.563299
AACGCAG211900.028.4132776
TATCAAC219450.028.393822
TACATGG314800.028.2040042
ACATGGG310450.027.3414343
TAGGCAT100550.027.0755945
CTAGTAC122500.026.3344233
ACGCAGA222250.026.0325577
CTAGGCA108550.025.7755174
TCAACGC229550.025.709094
TCTAACG37000.025.6739792
Produced by FastQC (version 0.11.5)