FastQCFastQC Report
Thu 26 May 2016
ERR580014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8408757
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92180.109623812413654No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108400.036.9490621
GTATCAA176600.036.4689251
CAACGCA179600.032.9213035
GTACTAG74400.031.9088761
ATCAACG191300.031.0552333
GGGCACC90400.029.06700794
TATCAAC222150.028.9437892
TAGTACT105450.028.7929424
GTACATG307850.028.4184441
AACGCAG218100.028.186046
CTGTGCG106650.028.115419
TACATGG313350.027.6446592
ACATGGG306550.026.6931693
TCAACGC232550.026.2538454
ACGCAGA228700.025.831597
CTAGTAC121900.025.678713
TAGGCAT97700.025.5929155
CTAACGC37000.025.2786623
TCTAACG37700.025.0593812
TAACGCC37700.025.0584874
Produced by FastQC (version 0.11.5)